氨基葡萄糖和硫酸软骨素预防治疗骨质疏松的协同作用

摘要

Objective To investigate the synergic effect of glucosamine hydrochlorid and chondroitin sulfate ( GHC ) in the prevention and treatment of osteoporosis.Methods The body weights ( BWs ) , the femur weights, the bone mineral density ( BMD) , and the calcium content ( Ca%) of the femur of the Wistar rats were determined.All the data were compared with those in calcium carbonate alone treated group ( positive control ) . The dosage used was the amount equal to the 5-fold higher recommended supplements for humans (GHC-5 group, 250 mg/kg per day), 10-fold higher (GHC-10 group, 500 mg/kg per day), and 30-fold higher (GHC-30 group, 1500 mg/kg per day).Rats in each group were fed with a low-calcium diet (0.15%Ca) and deionized water with GHC (GHC-5, GHC-10, GHC-30) or without GHC (negative control and positive control, the amount of dietary calcium was equal to GHC-30 group, 203 mg/kg).Results After 12-week treatment, the femur weight, BMD, and the femur Ca%in GHC-10 group increased significantly compared with those in negative control group.However the results of GHC-30 group were higher than those in positive group.Conclusion The present observation concurrents with earlier reports showing the synergic effect of GHC in the treatment of osteoporosis for human beings.%目的：评价氨基葡萄糖和硫酸软骨素在预防和治疗骨质疏松方面的协同作用。方法通过测定Wistar大鼠的体重、股骨重量、骨密度以及骨钙含量，并与单独饲喂碳酸钙组（阳性对照组）进行对照。饲喂的剂量分别相当于人体推荐剂量的5倍（ GHC－5组，每日250 mg／kg・BW），10倍（ GHC－10组剂量，每日500 mg／kg・BW）和30倍（ GHC－30组，每日1500 mg／kg・BW）。然后分别测定并比较给药组、阳性对照和阴性对照的股骨重，骨矿物质密度（ BMD）和钙的含量（钙％）。各组均喂低钙基础饲料（0．15％），自由饮用去离子水。碳酸钙对照组的每日食用钙含量与高剂量组相同，即203mg／kg・BW，低钙对照组以等体积的去离子水代替受试物溶液灌胃。结果实验12周后，GHC－10组的股骨重量、骨密度和骨钙含量与阴性对照组相比有显著提高，而GHC－30组的3个测试指标比阳性对照组显著增加。结论实验结果以及文献报道均表明盐酸氨基葡萄糖和硫酸软骨素对人类的骨质疏松症治疗具有协同效益。