绝经前后妇女 OPG 基因多态性与骨密度相关性研究

摘要

Objective To explore the relationship between single nucleotide polymorphism ( SNP) of osteoprotegerin ( OPG) and bone mineral density ( BMD) in pre-and post-menopausal women.Methods A cross-sectional study was conducted in 108 premenopausal and 127 postmenopausal women.Six genotypes of OPG were determined using chip-based matrix-assisted laser desorption ionization time-of-flight mass spectrometry.BMD of the lumbar spine, hip, and the femoral neck was evaluated using dual energy X-ray absorptiometry.Results BMD of the femoral neck in SNP rs6993813TT patients was significantly higher than that in TC/CC type patients (P<0.05).BMD of all parts in CC type patients was lower than that in TC/TT patients.BMD of the femoral neck in SNP rs4355801GG patients was significantly higher than that in AG/AA type patients (P<0.05).BMD of the femoral neck in SNP rs1032129AA patients was significantly higher than that in AC/CC type patients (P<0.05).BMD of the femoral neck in SNP rs2073618CC patients was significantly higher than that in GG/GC type patients (P<0.05).Conclusion The observed risk alleles of rs6993813, rs4355801, rs1032129, and rs2073618 in the present study were C, A, C, and G, respectively.By contrast, the protective alleles were T, G, A, and C, respectively.Women with protective alleles had higher BMD than women with risk alleles.The detailed mechanism needs further work to clarify.%目的：分析OPG基因的单核苷酸多态性（ SNP）与绝经前后妇女骨密度的关系。方法在235名绝经前后妇女中，采用基质辅助激光解吸电离飞行时间质谱技术对6个护骨素（ OPG）基因的SNP进行分型。应用双能X线骨密度仪测定腰椎、髋部和股骨颈骨密度（BMD）。结果 rs6993813TT基因型股骨颈BMD显著高于CC型，CC型各部位的BMD均低于TC／TT型（P＜0．05）。 rs4355801GG型股骨颈的BMD高于AG／AA型（P＜0．05）。 rs1032129 AA型股骨颈的BMD高于AC／CC型（P＜0．05）。 rs2073618的CC基因型股骨颈的BMD显著高于GG／GC型（P＜0．05）。结论 rs6993813， rs4355801， rs1032129和rs2073618均与中国绝经前后女性BMD有关，其危险基因分别为C， A， C和G，而T，G，A和C则具有保护作用，含有保护基因者骨密度高于含有危险基因者，但确切的机制尚需在今后工作中进一步研究阐明。