麝香对骨缺损模型大鼠HMGB1和BMP2表达的影响

摘要

目的 探讨麝香对高迁移率族蛋白1(highmobilitygroupbox-1protein,HMGB1)和骨形态发生蛋白2(bone morphogenetic protein-2,BMP2)在颅骨骨缺损模型大鼠组织中表达的变化.方法 SD大鼠300只,建立颅骨骨缺损模型.随机分为对照组和给药组,每组150只,再将这两个组按照第7、14和28天分为3个小组,每组50只.给药组按42 mg/kg给予天然麝香灌胃,对照组灌服等体积的生理盐水,均每日1次.第7、14 和28 天采用蛋白印迹法检测HMGB1和BMP2的表达.结果 给药组HMGB1、BMP2在第7天的表达均达到峰值(P＜0.05),第14 天时降低,第28 天时表达最低;与对照组比较,第7 天 HMGB1、BMP2表达均有统计学意义(P＜0.05);给药组第7 和14 天HMGB1的表达明显高于本组第28 天(P＜0.05).结论 麝香促进大鼠HMGB1、BMP2的表达增加,可能是颅骨骨缺损处的愈合原因之一.%Objective To discuss the expression of high mobility group box 1 protein(HMGB1)and bone morphogenetic protein 2(BMP2)in rat models of calvarial bone defect.Methods Skull bone defect model was established in 300 Sprague-Dawley rats and the rats were randomly divided into control group and treatment group,with 150 rats in each group.Each group was divided into 3 groups of 7 d,14 d and 28 d,with 50 rats in each group.The rats in the treatment group were given 42 mg/kg of natural musk,and the control group was given the same amount of normal saline,once per day.The expression of HMGB1 and BMP2 was detected by Western blotting at 7 d,14 d and 28 d.Results The expression of HMGB1 and BMP2 in the treatment group reached the peak on the 7th day(P＜0.05),decreased on the 14th day and was the lowest on the 28th day.Compared with the control group,the expression of HMGB1 and BMP2 on the 7th day was significantly different(P＜0.05).In the treatment group,the expression of HMGB1 on the 7th and 14th day was significantly higher than that of the 28th day(P＜0.05).Conclusion Musk increased the expression of HMGB1 and BMP2 in rats,which may be one of the causes of skull bone defect healing.