血清肿瘤坏死因子α水平在大鼠心肺移植术后早期急性排斥反应诊断中的意义

摘要

采用单一行供体升主动脉与受体腹主动脉端侧吻合的简化手术方法,建立较为稳定的大鼠异位心肺移植模型.分对照组、环孢素组及不用药组,分别于术后3、5、7天应用酶联免疫双抗体夹心法测定受体大鼠血清肿瘤坏死因子α(TNFα)水平,并于处死后行病理组织学检查.结果不用药组TNFa的水平较对照组和环孢素组高(P＜0.01,P＜0.05),病理切片检查不用药组的排斥反应程度明显高于其它两组.结果表明,在心肺移植排斥反应过程中,受体血清中TNFα的水平升高,这可作为诊断心肺移植术后排斥反应的一个有效指标.%In this study an acute rejecting model of heterotopic rat heart-lung transplantation was established by using simplified technique of end to side anastomosis of donor ascending aorta with recipient abdominal aorta.The measurements of tumor necrosis factor alpha(TNF-α)in serum of rats by Sandwich-ELISA method were taken on the 3rd,5th,7th days respectively after operation.The results of this study with histological examination showed that TNF-α serum levels were increased during acute rejection episodes and it might be a useful indicator for diagnosis of allograft rejection.