增生性糖尿病视网膜病变和增生性玻璃体视网膜病变患者玻璃体中TGF-β2的表达及意义

摘要

目的 通过定量测定增生性玻璃体视网膜疾病患者玻璃体中转化生长因子β2(TGF-β2)含量,探讨TGF-β2在增生性玻璃体视网膜疾病发生发展过程中的作用.方法 实验研究.收集行玻璃体切除手术61例(61眼)增生性玻璃体视网膜疾病患者玻璃体标本,其中增生性糖尿病视网膜病变(PDR)组37例(37眼)、增生性玻璃体视网膜病变(PVR)组24例(24眼),正常对照组8例(8眼)采用酶联免疫吸附测定法(ELISA)定量检测PDR患者、PVR患者和正常对照组玻璃体TGF-β2含量.结果 PDR组、PVR组及正常对照组玻璃体TGF-β2含量分别为(22.71 ±2.32) ng/ml,(21.28± 1.79) ng/ml,(19.00±0.62)ng/ml,PDR组、PVR组玻璃体TGF-β2含量均高于正常对照组,差异有统计学意义(F=7.756,P＜0.01).PDR组玻璃体TGF-β2含量高于PVR组,差异有统计学意义(P＜0.05).PDR组TGF-β2含量Ⅵ期高于Ⅴ期,差异有统计学意义(P＜0.01).PVR组TGF-β2含量D级高于C级,差异有统计学意义(P＜0.01).PDR组TGF-β2含量与眼病史呈正相关(r=0.705,P＜0.01).PVR组TGF-β2含量与眼病史呈正相关(r=0.934,P＜0.01).结论 TGF-β2可能在增生性玻璃体视网膜疾病的发生发展中起到重要作用.%Objective To observe the expression of transforming growth factor-β2 (TGF-β2) in the vitreous body of patients with proliferative vitreoretinal diseases; to investigate the role of TGF-β2in the pathogenesis of proliferative vitreoretinal diseases.Methods In an experimental study,vitreous specimens were obtained during vitrectomy from 61 patients (61 eyes) with proliferative vitreoretinal diseases.The vitreous specimens were obtained from 37 eyes with proliferative diabetic retinopathy (PDR) and 24 eyes with proliferative vitreoretinopathy (PVR).Eight vitreous fluid samples were obtained from a normal control group.The concentrations of TGF-β2 in the vitreous body were detected by enzyme-linked immunosorbent assay (ELISA).Results The concentrations of TGF-β2 in the vitreous bodies of eyes with PDR,PVR and the normal controls were 22.71±2.32 ng/ml,21.28±1.7)ng/ml,and 19.00±0.62 ng/ml,respectively.Intravitreous concentrations of TGF-β2 were higher in patients with PDR and PVR than in the control group.The difference was statistically significant (F=7.756,P＜0.01).The concentration of TGF-β2 in the vitreous body from eyes of PDR patients was clearly higher than concentrations in PVR patients.The difference was statistically significant (P＜0.05).The concentrations of TGF-β2 in the vitreous body were higher in level Ⅵ of the PDR group in comparison to level Ⅴ of the PDR group.The difference was statistically significant (P＜0.01).The concentrations of TGF-β2 in the vitreous body were higher in the D class of the PVR group in comparison to the C class.The difference was statistically significant (P＜0.01).The concentrations of TGF-β2 in the vitreous bodies were positively correlated with the duration of the diseases:the correlation for the PDR group was r=0.705 (P＜0.01) and the correlation for the PVR group was r=0.934(P＜0.01).Conclusion TGF-β2 may play an important role in the pathogenesis and development of proliferative vitreoretinal diseases.