结膜上皮干细胞的体外培养与分化的研究进展

摘要

Conjunctiva epithelial stem cells are bipotent cells giving rise to both conjunctival epithelial cells and goblet cells. They play a central role in ocular surface homeostasis and wound repair, and also have great capacities of self-renewal, proliferation and differentiation. As a result, conjunctival epithelial stem cells are main resources of seed cells for tissue engineering conjunctiva. To review the current research of conjunctival epithelia stem cells such as their features, distribution, ways to identify stem cells,in vitro culture and induced differentiation to goblet cells.%结膜上皮干细胞可以分化为结膜上皮细胞和杯状细胞,在维持正常眼表与损伤修复中具有关键作用,具有较强的自我更新和增殖分化能力.因此,结膜上皮干细胞是结膜组织工程种子细胞的重要来源.现就结膜上皮干细胞的生物特性、在眼表的分布、常用的检测方法、体外培养情况、诱导分化为杯状细胞的研究进展进行分析.