中药松友饮对裸鼠体内化疗后残余肝癌的作用

摘要

目的 探讨中药松友饮对化疗后残余肝癌细胞侵袭转移潜能的影响及相关机制.方法 采用人肝癌MHCC97L细胞构建奥沙利铂化疗后的残余肝癌裸鼠原位移植模型.成瘤后,实验组裸鼠以2.1 g/kg、4.2 g/kg和8.4 g/kg的松友饮灌胃,对照组裸鼠灌服生理盐水,检测松友饮对化疗后残余肝癌生长、转移及对裸鼠生存时间的影响.结果 松友饮可有效降低化疗后残余肝癌的肺转移能力,对照组、2.1 g/kg松友饮治疗组、4.2 g/kg松友饮治疗组和8.4 g/kg松友饮治疗组裸鼠的肺转移率分别为86.7％(13/15)、73.3％(11/15)、40.0％ (6/15)和20.0％(3/15),与对照组比较,4.2 g/kg松友饮治疗组和8.4g/kg松友饮治疗组裸鼠肺转移率显著下降(P=0.021,P=0.001).松友饮可延长残余肝癌再接种裸鼠的生存时间,对照组、2.1 g/kg松友饮治疗组、4.2 g/kg松友饮治疗组和8.4g/kg松友饮治疗组裸鼠的生存时间分别为(53.83 ±4.71)d、(56.50 ±6.09)d、(66.67 ±5.61)d和(81.17±7.36)d,与对照组比较,4.2 g/kg松友饮治疗组和8.4 g/kg松友饮治疗组裸鼠生存时间延长(P =0.002,P=0.001).松友饮治疗后,残余肝癌组织的上皮-间质转化(EMT)现象显著减少,E-cadherin表达恢复,钙黏蛋白转换现象逆转.结论 松友饮可有效抑制化疗后残余肝癌组织的EMT,降低残余肝癌组织侵袭转移潜能,延长残余肝癌再接种裸鼠的生存时间.松友饮与化疗药物合用有可能进一步提高肝癌化疗的疗效.%Objective To investigate the effects of a Chinese herbal extract Songyou Yin on residual hepatocellular carcinoma after chemotherapy in nude mice and the relevant mechanisms.Methods Orthotopic nude mouse models bearing residual hepatocellular carcinoma after chemotherapy was established using human liver carcinoma MHCC97L cells.Three different doses of Songyon Yin (2.1 g/kg,4.2 g/kg and 8.4 g/kg) were administered to the mice in the trial groups by intragastric gavage,respectively.The mice in the control group were administered physiological saline.The tumor growth,metastasis and survival in the mice of each group were recorded.The corresponding mechanisms were studied.Results The pulmonary metastasis rates of the control group and 2.1g/kg,4.2g/kg,8.4g/kg Songyou Yin treatment group were 86.7％,73.3％,40.0％,and 20.0％,respectively,and the survivals of these groups were 53.83 ± 4.71,56.50 ± 6.09,66.67 ± 5.61,81.17 ± 7.36 days,respectively.Compared with the mice in the control group,mice in the 4.2 g/kg,8.4 g/kg Songyou Yin treatment groups had a lower pulmonary metastasis rate (P =0.021 and P =0.001,respectively) and longer survival (P =0.002 and P =0.001,respectively).A restoration of E-cadherin expression and a concomitant reduction of N-cadherin expression were detected in the tumors of the 4.2 g / kg and 8.4 g / kg Songyou Yin treatment groups.Conclusions Songyou Yin effectively inhibits the invasion and metastasis of the residual hepatocellular carcinoma after chemotherapy in nude mice through attenuating the epithelia-mesenchymal transition and prolongs the survival.Songyon Yin may have potential to promote the efficacy of chemotherapy in hepatocellular carcinoma.