珍珠豆型花生品种白沙1016核型分析

摘要

珍珠豆型花生白沙1016是重要的花生种质资源.利用高度保守的重复序列5S和45SrDNA作探针对白沙1016根尖细胞中期染色体进行荧光原位杂交(FISH)分析,发现两探针在白沙1016的12条染色体上能产生杂交信号,其中1对染色体长臂与短臂、2对染色体短臂、3对染色体长臂具有杂交信号.综合DAPI+染色带纹和染色体长度、臂比、面积等特征,可以将白沙1016大部分染色体区分开,为白沙1016的育种利用提供了染色体遗传分析基础.%Spanish peanut variety (Arachis hypogaea L.var.vulgaris Harz) Baisha 1016 was a popular cultivar and an elite germplasm resource.Using the highly conserved repeat sequences of 5S rDNA and 45S rDNA as probes,FISH (fluorescence in situ hybridization) analysis of metaphase chromosomes in cells from peanut root tips were conducted.Hybridization signals were observed in 12 chromosomes including in both long and short arms of one pair of chromosome,short arms of two pairs of chromosomes,and long arms of three pairs of chromosomes.Most of the chromosomes of Baisha 1016 could be distinguished and characterized based on the hybridization signals combined with DAPI+ bands and length,arm ratio and area of chromosomes.The results provided the genetic basis of chromosome analysis for utilization of Baisha 1016 in further breeding programs.