胶体金免疫层析法快速定量分析粮油农产品中黄曲霉毒素B1

摘要

采用自主研制的黄曲霉毒素B1胶体金免疫定量检测卡，建立花生、玉米、大米、小麦等粮油农产品中黄曲霉毒素B1的定量分析方法，4种样品检测的线性范围为1．0～20．0μg/kg，R2＞0．97，方法的定量限为1．0μg/kg，样品加标回收率为75％～106％，RSD＜20％。胶体金免疫层析法与免疫亲和柱净化-HPLC 法相比，相对误差＜15％，具有简便快速、灵敏度高、重现性好等特点，适用于粮油农产品及制品中黄曲霉毒素B1筛查，样品检测时间只需15min，检测成本低于其他方法。%A rapid and quantitative detection method based on colloid gold immuno-chromatographic method was developed to analyze aflatoxin B1 in several plant samples such as peanut,corn,rice,wheat.The quantitative detection of aflatoxin B1 in these samples could be completed within 15 minutes.The dynamic linear range of the calibration curves was 1.0 μg/kg-20.0 μg/kg (R2 >0.97),and the limit of quantification was up to 1.0 μg/kg,with the recovery rate of 75%-106%(RSD<20%).Compared with immuno-affinity column purification-liquid chromatography,the relative standard deviation (RSD)was less than 15%.This detection method cost less than other methods,and with the advantage of shorter-time and higher-sensitivity,this method could be used for aflatoxin B1 screening in plant agro-products.