大豆GmNCED 1基因的克隆及表达模式分析

摘要

Soybean (Glycine max)is one of the important oil crops in the world.The yield of soybean is strongly affected by environmental stress such as salinity and dehydration.Here we cloned the 9-cis-epoxycarote-noid dioxygenase gene (named GmNCED1 )from soybean (Hanjian 1 )which had stronger adaptability and toler-ance to abiotic stresses.The open reading frame of GmNCED1 was 1 836 bp,encoding 6 1 1 amino acids.Sequence analysis and homology comparison of GmNCED1 showed that it had highest homology with dicotyledonous plants (peanuts,pea,etc)with more than 85% homologies.At the same time,we analyzed the expression profiling of GmNCED1 during various abiotic stresses using qRT-PCR.The result showed that the expression of GmNCED1 could be induced by salt,drought,NAA,and ABA treatments in leaves and roots.This study explained the func-tion of GmNCED1 in abiotic stresses and provided a candidate gene for genetically engineered crops.%本研究以抗逆性强的大豆旱碱一号为材料，首次从中克隆了编码9-顺式-环氧类胡萝卜素双加氧酶（9-cis-epoxycarotenoid dioxygenase，NCED）的GmNCED1基因全长cDNA片段，该基因编码区含有1836个核苷酸，编码611个氨基酸。通过同源性比对分析发现GmNCED1与花生、菜豆等双子叶豆科植物NCED的同源性高达84％以上。同时，利用荧光定量PCR分析发现高盐、低温、干旱、外源ABA以及NAA处理均可以诱导该基因在大豆叶片及根中表达。本研究初步揭示GmNCED1基因在植物逆境胁迫中的作用，为基因工程育种提供优质的候选基因。