髓样细胞表达触发受体在缺血性视神经病变中的表达

摘要

Objective To observe the expression of triggering receptor expressed on myeloid cells (TREM),Caspase-3 and interleukin (IL)-6 in optic nerve tissue of ischemic optic neuropathy (ION).Methods Twenty Sprague-Dawley rats were randomly divided into control group and model group,10 rats in each group.The permanent ligation of bilateral internal carotid arteries (BICA) was performed for 14 days to establish subacute ION model as model group.The control group were separated BICA without ligation.The expressions of TREM-1,TREM-2,Caspase-3 and IL-6 in rat retina were detected by reverse transcription PCR and Western blot,respectively.Results Compared with the control group,the expressions of TREM-1,Caspase-3,IL-6mRNA (t=6.058,7.86,6.055) and protein (t=9.671,9.524,14.501) in the optic nerve tissue of the model group were increased,while the expression ofTREM-2 mRNA and protein (t=9.283) was decreased,and the difference was statistically significant (P＜0.05).Conclusion In ischemic optic nerve tissue,TREM-1 mRNA and protein were significantly expressed,the expressions of TREM-2 mRNA and protein decreased significantly.%目的 观察缺廊性视神经病变视神经组织中髓样细胞表达触发受体(TREM)、半胱氨酸蛋白酶(Caspase)-3、白细胞介素(IL)-6的表达.方法 Sprague Dawley大鼠20只随机分为对照组、模型组,每组均为10只大鼠.模型组大鼠行双侧颈内动脉结扎建立亚急性缺血性视神经病变动物模型;对照组大鼠游离双侧颈总动脉,但不结扎.建模后14d,实时定量聚合酶链反应、蛋白质免疫印迹法检测大鼠视神经组织中TREM-l、TREM-2、Caspase-3、IL-6基因及蛋白表达.结果 与对照组比较,模型组大鼠视神经组织中TREM-l(tmRNA=6.058,t蛋白=9.671)、Caspase-3(tmRNA=7.86,t蛋白=9.524)、IL-6(tmRNA=6.055,t蛋白=14.501)mRNA和蛋白表达升高,TREM-2(tmRNA=6.992,t蛋白=9.283)mRNA和蛋白表达降低,差异均有统计学意义(P＜0.05).结论 TREM-1基因和蛋白在缺血性视神经组织中显著表达;TREM-2基因和蛋白显著下降.