原因不明性原发不孕患者子宫内膜白血病抑制因子基因的表达

摘要

Objective　To investigate the expression of endometrial leukaemia inhibitory factor（LIF） gene in patients with unexplained infertility. Methods　 By a quantitative reverse transcription-polymerase chain reaction (RT-PCR)，the expression of LIF gene on endometrium during mid-luteal phase was detected in 35 unexplained infertility (infertility group) cases and 20 infertile cases due to tubal obstruction or male factor (control group). Results　The level of LIF mRNA expression on endometrium during mid-luteal phase in infertility group was 0.448±0.239，significantly lower than those in the control group （1.093±0.761，P<0.01). Conclusions　Our findings suggested LIF might play an important role in the process of implantation. The decreased expression of LIF gene might be one of the major causes of unexplained infertility.%目的探讨白血病抑制因子（LIF）基因在子宫内膜的表达与原因不明性原发不孕的关系。方法采用逆转录聚合酶链反应（RT-PCR）技术，对35例原因不明性原发不孕患者（病例组）和20例继发不孕患者（对组照）的黄体中期子宫内膜行LIF mRNA检测。结果对照组患者黄体中期LIF mRNA表达水平为1.093±0.761（±s，下同），病例组患者为0.448±0.239，两组相比，差异有极显著性（P<0.01）。结论 LIF可能参与了胚泡着床过程。LIF的基因表达缺陷或减弱，可能是导致原因不明性原发不孕的原因之一。