黑果枸杞内生真菌E21菌株次生代谢产物的研究

摘要

Objective: To study the secondary metabolites of endophytic fungus E21 from the root of Lyci-um ruthenicum. Methods: The extract was taken from its solid-state fermentation, which can secrete antibacterial metabolites. Results: The strain E21 was identified as Irpex lacteus by its morphological characters and internal transcribed spacer (ITS) sequence analyses. Four compounds were isolated by column chromatography method and recrystallization technique, and identified as monacolin K, dehydromonacolin K, (22E ,24R) -5α, 8α-epidioxyerg-osta-6 ,22-dien-3β-ol and ergosterol. Conclusion: Monacolin K and dehydromonacolin K are first reported from Irpex lacteus. Monacolin K shows a stronger inhibition to the growth of Gram negative and Gram positive bacteria; however dehydromonacolin K shows a moderate inhibition to the growth of Bacillus subtilis.%目的:研究黑果枸杞根部的内生真菌E21次生代谢产物.方法:利用固体大米培养基对该菌株进行发酵培养,采用柱色谱以及重结晶等方法分离化合物.结果:经形态学特征及ITS序列分析结果鉴定菌株E21为白耙齿菌Irpex lacteus.从其发酵物中分离得到4个化合物,分别为monacolin K,dehydromonacolin K,5α,8α-环二氧麦角甾-6,22-二烯-31-醇和麦角甾醇,其中monacolin K和dehydromonacolin K首次从白耙齿菌Irpex lacteus中分离得到.结论:monacolin K对革兰阴性菌和革兰阳性菌都具有很好的抑菌活性,而dehydromonacolin K仅对枯草芽孢杆菌具有一定的抑菌活性.