一株黑果枸杞内生真菌RML6的黄色素稳定性研究

摘要

Endophytic fungi in plants have the potential of producing natural active substances and it is one of the sources of natural pigments.In this study,endophytic fungi RML6 is screened out from the leaves of Lycium ruthenicum Murr.,and the strain is identified as Coprinellus radians,The yellow pigment which produced at this strain has the highest absorption peak at 341 nm.The stability of the yellow pigment which produced by the endophytic fungus RML6 of Lycium ruthenicum Murr was investigated from the aspects of temperature,pH,light,metal ion,oxidant and reducing agent.The results show that the pigment has no significant change in the light absorption value of yellow pigment for 8 hours under natural light,but it is less sensitive to UV storage.It is not affected much at pH 6-10 and 20-100 ℃.In the metal ions,Na+,k +,Mg2 +,Cu2 +,Fe3 + have color protection or burnish effect on the pigment,Zn2 +,Mn2 +,Ca2 + have a significant effect on the pigment.H2O2 and NaHSO3 have strong destructive power to the pigment though several common additives have no effect on the pigment.Meanwhile,sucrose also has a significant hyperchromic effect on it.So the pigment can be used for a wide range of applications.%植物内生真菌具有产天然活性物质的潜能,亦是天然色素的来源之一,本研究从黑枸杞叶部分离筛选得到产黄色素的内生真菌RML6,经鉴定该菌为Coprinellus radians,该菌株所产黄色素在341 nm波长处有最大吸收峰.从温度、pH、光照、金属离子、氧化剂与还原剂等方面探究黑枸杞内生真菌RML6所产黄色素的稳定性.结果表明该色素在自然光下放置8h黄色素光吸收值无显著变化,但对紫外较为敏感应尽量避光存放;在pH 6～10,20～100℃下不受太大影响;金属离子中,Na+、K+、Mg2+、Cu2、Fe3对该色素有护色或增色作用,Zn2、Mn2、Ca2对色素有明显的影响;H2O2、NaHSO3对该色素有较强的破坏力;常见几种添加剂对色素无太大影响,同时蔗糖对其还具有明显的增色效应.该色素可广泛用于多个用途.