Objective To detect the change of MMP-3 in the prominent intervertebral disc tissue and blood in rats of intervertebral disc herniation to explore the feasibility of conservative treatment for intervertebral disc hernia -tion with MMP3-.Methods Forty SD rats(only 3 months of age) were randomly divided into normal control group , negative control group,experimental group 1 and experimental group 2, 10 in each group.The MMP-3 content in disc herniation organization and the blood were detected with enzyme -linked immunosorbent assay method after 45 days of experiment.Re sults (1)The expression of MMP-3 was significantly higher in the experimental group 1 and group 2 compared with that of normal control group (P<0.05);(2)The express of MMP-3 was significantly higher in group 1 compared with that of group 2(P<0.05); (3)the concentration of MMP-3 between intervertebral disc tissue and blood in group 1 and group 2, had a linearly relating, and the correlation coefficients were 0.835, and 0.924 ( P<0.05 ) .Conclusion The content of MMP-3 in the blood rises in the process of intervertebral disc hernia -tion resorption ,and content of MMP-3 in local intervertebral disc tissue is highly correlated with the level of that in the blood, idea of nonsurgical treatment by local injections with MMP-3 to cure intervertebral disc herniation may not come true in the clinical use .%目的：检测大鼠突出椎间盘组织及血液中基质金属蛋白酶3（ MMP－3）含量的变化，探讨MMP－3保守治疗椎间盘突出症的可行性。方法随机将40只3月龄SD大鼠，分为正常对照组、阴性对照组、实验组1及实验组2，每组10只。造模45 d后，用ELISA法检测椎间盘突出组织及血液中MMP3－含量。结果（1）与正常对照组相比，实验组1、2的椎间盘组织及血液MMP－3浓度上升，差异有统计学意义（P＜0．05）。（2）实验组1椎间盘组织及血液MMP－3浓度较实验组2明显升高，差异有统计学意义（P＜0．05）。（3）实验组1、2椎间盘组织中 MMP－3浓度的变化与血液中MMP－3相关系数分别为0．835和0．924，均P＜0．05。结论椎间盘突出再吸收过程中，血液中MMP－3含量上升，且局部椎间盘组织与血液中MMP－3的含量高度正相关，临床上运用MMP－3局部注射非手术方法治疗椎间盘突出症的设想可能行不通。
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