可降解水凝胶栓塞动脉瘤的实验研究

摘要

Objective To evaluate the feasibility of injectable thermosetting chitosan/glycerophosphate(C/GP)hydrogel as the embolism material of aneurysms.Method 2%chitosan solution and 56%glycerophosphate solution were mixed in different volume ratios.The pH,colloidization time in 37℃and mechanic strength of the mixed solution were detected respectively to determine the best ratio.The intracatheter operation of the C/GP hydrogel by embolizing aneurysm models was evaluated.The C/GP hydrogel histocompatibility was evaluated by embolizing rabbit ear arteries. Finally,we embolized rabbit aneurysm models with this C/GP gel.Results When the ratio of C/GP was 7:1 at pH 7.28,the colloidization time was(260±18)seconds and the mechanic strength chould reach 14 kPa.In the experiment of embolizing aneurysm models in vitro,the C/GP gel could arrive in aneurysms through catheter and reach solid state.In the experiment of embolizing rabbit ear arteries,the modest inflammation was observed.This effect peak was found in 1 week,and gradually resolution in 2-3 weeks.In 4-8 weeks,granulation tissue and slight degradation of C/GP hydrogel could be observed.In the embolism operation of rabbit aneurysms,the aneurysms were disappeared in angiography.In the HE stain slice 3 days after operation,the C/GP hydrogel in aneurysm caves and normal endomembrane cells were observed.There was no significant flame cell in aneurysm walls.Conclusions In short term view,C/GP hydrogel could be used to embolize aneurysms.%目的 探讨壳聚糖/甘油磷酸钠水凝胶作为动脉瘤栓塞材料的可行性.方法 配制2%壳聚糖与56%甘油磷酸钠溶液,不同比例混合,测定混合液pH、37℃成胶时间及力学强度,选择最佳比例.体外栓塞动脉瘤模型,评价导管内操控性;通过兔耳中动脉栓塞评价组织相容性.最终栓塞兔动脉瘤模型.结果 当壳聚糖/甘油磷酸钠在7∶1时,pH值为7.28,成胶时间为(260±18)s,力学强度可达14 kPa.体外动脉瘤模型栓塞见壳聚糖/甘油磷酸钠水凝胶可通过导管在动脉瘤内成胶.兔耳中动脉栓塞后3 d,血管周围可见少量炎性细胞浸润,1周时达到高峰,2-3周逐渐消退,4-8周形成肉芽组织,并可见壳聚糖凝胶部分降解.兔动脉瘤栓塞术中见动脉瘤不显影,术后3 d病理见动脉瘤被凝胶栓塞,内膜细胞形态正常,动脉壁内未见明显炎性细胞浸润.结论 短期观察,壳聚糖/甘油磷酸钠水凝胶可用于动脉瘤栓塞.