ω-3多不饱和脂肪酸对慢性轻度应激鼠海马基因表达的影响

摘要

Objective To study the effect of omega-3 polyunsaturated fatty acids (to-3PUFAs)on gene expression profile of the hippocampus in chronic mild stress rats using microarray. Methods All rats were divided into three groups (6 rats per group) according to the table of random digit: research group (treateol with (0-3PUFAs 3.1 ml-kg-1-d-1 + CMS), model control group (treateol with saline + CMS) and normal control group. Weight and sucrose intake were examined every week. The hippocampus tissues from research group (3rats randomly chosen) and model control group (all rats) was used to assess the impact ofω-3PUFAs on gene expression profile of the hippocampus using a cDNA microarray with 10,891 distinct rat genes. Results There were no difference in weight between research group and model control group throughout the whole experiment (P > 0.05). On week 7 and 8, sucrose intake were higher in research group than in model control group (P < 0.01), but were no different between research group and normal control group (P > 0.05). Thirty-five genes had altered change in to-3PUFAs treated rats. Among them, 12 genes were upregu-lated and 23 genes was downregulated. Those genes include transport protein gene (Slcl7a9、Rtp4、Slcl5a4、Kpnbl、 Abcb7), signal transduction gene(Jun、Strada), voltage dependence ion channel gene(Vdac2)and immune function protein gene(IgE receptor (3 subunit、 IgG2a heavy chain). Conclusions ω-3PUFAs can improve the depressant-like behavior in chronic mild stress rats which may invlove many genes including transport protein, signal transduction, ion channel, and immune genes and some genes with ambiguous function.%目的 采用基因芯片技术筛选ω-3多不饱和脂肪酸(omega-3 polyunsaturated fatty acids,ω-3PUFAs)对慢性轻度应激(chronic mild stress,CMS)抑郁大鼠海马作用的相关基因.方法 将大鼠根据随机数字表分为3组(6只/组)给予相应处理8周:研究组(ω-3PUFAs 3.1 ml·kg-1·d-1+CMS),模型对照组(生理盐水+CMS)和正常对照组.每周进行行为学指标(体重和糖水摄入量)的测定.采用大鼠10,891条全基因组cDNA芯片,检测研究组(随机取3只大鼠)和模型对照组(全部大鼠)海马的差异表达基因,评价ω-3PUFAs对基因表达的影响.结果 整个试验过程,ω-3PUFAs对体重没有显著影响(P>0.05),试验第7、8周,研究组糖水摄入量高于模型对照组(P<0.01),且与正常对照组无差异(P>0.05).与模型对照组比较.研究组共35条基因有差异表达,其中12条基因上调表达,23条基因下调表达.其中主要包括:转运蛋白基因(Slc 17a9、Rtp4、Slc15a4、Kpnb1、Abcb7),信号转导通路基因(Jun、Strada),电压依赖性离子通道基因(Vdac2).免疫功能蛋白基因(IgE受体β亚单位、IgG2a重链)等.结论 ω-3PUFAs具有抗抑郁作用,其作用机制可能与对转运蛋白、信号转导通路、离子通道、免疫功能蛋白等多类基凶的表达调节有关.