SLC5A8基因在原发性肝癌组织中的表达及其与肝癌预后的关系

摘要

目的 探讨SLC5A8蛋白在肝癌组织中的表达及其与肝癌预后的关系.方法 选取石蜡包埋的肝癌、癌旁组织及正常肝组织标本各97例,采用免疫组化Elivisionplus方法检测SLC5A8蛋白的表达情况,分析SLC5A8在不同病理状态下的表达,并结合术后随访资料,研究SLC5A8基因与肝癌预后的关系.结果 97例肝癌标本中,SLC5A8蛋白在肝癌组织中表达明显低于其在癌旁组织及正常肝组织中的表达(P＜0.01).其中,SLC5A8蛋白在肿瘤直径≤5cm、组织分化程度良好/中等、无肝硬化、AFP≤400ng/ml、肿瘤分级Ⅰ～Ⅱ级的肝癌组织中的表达分别高于肿瘤直径＞5cm、组织分化程度差、有肝硬化、AFP＞400ng/ml、肿瘤分级Ⅲ～Ⅳ级的肝癌,有统计学差异(P＜0.05);而在不同年龄段、不同性别、不同HBsAg(阴性、阳性)的原发性肝癌中,SLC5A8蛋白的表达无统计学差异(P＞0.05).Kaplan-Meier生存曲线分析,SLC5A8高表达者预后比SLC5A8低表达者好,两者中位生存时间比较有统计学差异(P＜0.001).结论 SLC5A8基因表达降低或缺失在肝癌发生、发展中起到了一定的作用,合并肝硬化可能是肝癌发生的重要相关因素.SLC5A8蛋白高表达的患者术后生存时间相对较长,对原发性肝癌术后患者判断预后可能有一定的作用.%Objective To investigate the expression of SLC5 A8 protein in hepatocellular carcinoma(HCC) and its relationship with the prognosis of HCC. Methods 97 cases of paraffin-embedded hepatocellular carcinoma (HCC) were selected. The expression of SLC5A8 protein was detected by immunohistochemical Elivision plus method. The expression of SLC5A8 under different factors was analyzed. Combined with the postoperative follow-up data, the relationship between SLC5A8 gene and prognosis of HCC was studied. Results The expression of SLC5A8 protein in HCC tissues was significantly lower than that in paracancerous tissues and normal liver tissues (P＜0.01). The expression of SLC5A8 protein in HCC tissues with tumor diameter ≤5 cm, well / moderate differentiation, no cirrhosis, AFP ≤400 ng / ml, tumor grade Ⅰ～Ⅱ was higher than that of tumor diameter ＞5 cm, poor differentiation, liver cirrhosis, AFP ＞400 ng / ml, and tumor grade Ⅲ～Ⅳ (P＜ 0.05). There was no significant difference in the expression of SLC5A8 protein in primary hepatocellular carcinoma(HCC) with different ages, genders and HBsAg (negative or positive) (P＞ 0.05). Kaplan-Meier survival curve analysis showed that the prognosis of patients with high expression of SLC5A8 was better than that of patients with low expression of SLC5A8, and there was significant difference in median survival time between the two groups (P＜0.001). Conclusion The decrease or deletion of SLC5A8 gene expression plays a certain role in the occurrence and development of HCC, while the liver cirrhosis may be an important related factor in the occurrence of HCC. The patients with high expression of SLC5A8 protein have a relatively long postoperative survival time, which may plays a important role in judging the prognosis of patients with primary HCC after operation.