铜绿假单胞菌生物被膜形成过程中多糖合成相关基因表达的研究

摘要

目的 了解铜绿假单胞菌(Pseudomonas aerugirtosa,Pa)生物被膜形成过程中多糖生物合成相关基因在生物被膜形成中的表达,探讨其在生物被膜形成中的调控作用.方法 分别收集非黏液型铜绿假单胞菌PAO1的浮游菌及生物被膜菌,用实时荧光定量RT-PCR的方法对基因的表达进行相对定量分析.结果 多糖合成相关基因pslA、algD.pelA的mRNA在生物被膜菌中的相对表达量均高于浮游菌.结论 .pslA、algD,pelA的表达与铜绿假单胞菌生物被膜形成密切相关,在生物被膜形成中具有重要作用.%Objective To investigate expression of polysaccharides biosynthetic genes in biofilm formation in Pseudomonas aeruginosa PAO1. Methods Real-time RT-PCR was used to quantify mRNA to analyze their mRNA level during planktonic growth and biofilm cells. Results Analysis of the relative ex-pression levels indicated that the level of transcripts of planktonic pslA, algD, pelA was significantly lower than that of biofilms sessile growth in all other form bacteria. The levels of transcripts of pslA, algD, pelA were the highest at the first day of biofilms development. Conclusion From the observations in the present study, transcripts of the pslA, algD, pelA involved biofilm development.