Objective To investigate the relationship between the expressions of maspin gene in colorectal membrane,and their roles in the occurrence,progression,metastasis and prognosis of colorectal adenocarcinoma.Methods Immunohistochemistry technique was used to detect the expression of maspin in tissues of normal controls,adenoma,colorectal adenocarcinoma primary tumor,liver metastasis,lymph-node metastasis respectively.Results The expressions of maspin in normal controls,adenoma and colorectal adenocarcinoma primary tumor are down-regulated successively; the expression of maspin in colorectal adenocarcinoma with metastasis is lower than that in colorectal adenocarcinoma without metastasis(P＜0.05) ; the down-regulation of maspin expression is significantly correlated with the depth of invasion,differentiation,liver metastasis and lymph node metastasis of colorectal adenocarcinoma(P＜0.01,P＜0.05,P＜0.01,P＜0.05).Conclusion The down-regulation of expression of maspin probably plays an important role in the occurrence,progression and metastasis of colorectal adenocarcinoma.maspin is probable a predictive index for reflecting the metastasis of colorectal adenocarcinoma and provide a clue for early diagnosis of metastasis of colorectal adenocarcinoma.%目的 检测maspin基因在结肠黏膜中的表达,探讨其与结肠癌发生、发展、转移及预后的关系.方法 应用免疫组织化学技术检测maspin在正常结肠黏膜、腺瘤、结肠癌原发灶、肝转移灶、淋巴结转移灶中的表达.结果 maspin在正常结肠黏膜、腺瘤、结肠癌原发灶中的表达依次下调;maspin在伴有转移的结肠癌原发灶中的表达低于不伴有转移的结肠癌原发灶中的表达(P＜0.05);maspin的表达下调与结肠癌浸润深度、分化程度、肝脏转移和淋巴结转移有关(P＜0.01,P＜0.05,P＜0.01,P＜0.05).结论 maspin表达下调在结肠癌的发生、发展及转移的过程中可能起到一定的作用.maspin可能成为判断结肠癌有无转移的预测指标,为结肠癌转移的早期诊断提供线索.
展开▼
