Maspin

摘要： 目的探讨SLeX和Maspin表达与乳腺浸润性导管癌(BIDC)临床病理指标及预后的关系。方法80例BIDC手术切除标本及30例距癌灶3 cm的癌旁乳腺组织,应用EliVision^(TM) plus免疫组织化学二步法检测SLeX和Maspin在癌组织及癌旁乳腺组织中的表达,分析SLeX和Maspin与病人年龄、TNM分期、分化等级、淋巴结宏转移、术后5年内复发转移等临床病理指标的相关性。结果癌组织SLeX和Maspin阳性率分别为86.3%(69/80)和47.5%(38/80),癌旁乳腺组织分别为20.0%(6/30)和93.3%(28/30),差异有统计学意义(均P0.05)。在TNM(T3+T4)期、低分化、有淋巴结宏转移和5年内复发转移病人中SLeX高表达,Maspin低表达,呈负相关(r=-0.33)。结论SLeX和Maspin与BIDC发生发展显著相关,SLeX表达上调或(和)Maspin表达下调均预示癌组织分化差、进展快、预后不良。

摘要： 目的探讨maspin、Cyclin-1与胃癌临床分期及血管内皮生长因子(VEGF)的相关性。方法选取2019年1月至2021年5月该院收治的240例胃癌手术患者作为研究对象,收集其癌组织及癌旁组织进行相关指标检测,采用免疫组织化学染色法检测胃癌组织及癌旁组织maspin、Cyclin-1的表达水平,采用酶联免疫吸附试验检测VEGF水平。结果胃癌组织中maspin阳性42例(17.50%),Cyclin-1阳性174例(72.50%);癌旁组织中maspin阳性198例(82.50%),Cyclin-1阳性66例(27.50%)。与胃癌组织比较,癌旁组织中maspin阳性表达较高,Cyclin-1阳性表达较低,差异均有统计学意义(P<0.05)。Ⅰ期胃癌患者maspin阳性21例(26.92%),Cyclin-1阳性36例(46.15%);Ⅱ期胃癌患者maspin阳性12例(21.05%),Cyclin-1阳性48例(84.21%);Ⅲ期胃癌患者maspin阳性9例(17.65%),Cyclin-1阳性42例(82.35%);Ⅳ期胃癌患者maspin阳性0例(0.00%),Cyclin-1阳性48例(88.89%)。各期胃癌患者maspin和Cyclin-1阳性表达情况比较,差异均有统计学意义(P<0.05)。Ⅰ期胃癌患者VEGF表达水平为(62.60±7.22)pg/mL,Ⅱ期胃癌患者为(85.20±15.25)pg/mL,Ⅲ期胃癌患者为(118.30±22.34)pg/mL,Ⅳ期胃癌患者为(140.30±30.56)pg/mL,各期胃癌患者VEGF表达水平比较,差异均有统计学意义(P<0.05)。maspin与VEGF呈负相关(r=-0.365,P<0.05),maspin与Cyclin-1呈负相关(r=-0.298,P<0.05),VEGF与Cyclin-1呈正相关(r=0.386,P<0.05)。结论maspin在胃癌组织中呈低表达,Cyclin-1在胃癌组织中呈高表达,且不同分期胃癌患者maspin、Cyclin-1及VEGF表达水平不同,通过检测maspin、Cyclin-1和VEGF表达水平,对胃癌诊断分期有重要作用。

摘要： 目的:研究扭曲蛋白Twist和maspin在典型子宫内膜增生、不典型子宫内膜增生和子宫内膜样腺癌组织中的表达.方法:应用免疫组化检测40例典型子宫内膜增生、40例不典型子宫内膜增生和80例子宫内膜样腺癌组织中Twist、maspin的表达.结果:在典型子宫内膜增生、不典型子宫内膜增生和子宫内膜样腺癌组织中Twist、maspin阳性率分别为10％、37.5％和52.5％,12.5％、42.5％和43.8％.Twist在不典型子宫内膜增生和子宫内膜样腺癌组织中的表达均高于典型子宫内膜增生组织,差异有统计学意义(P<0.05).子宫内膜样腺癌组织中,Twist与组织学分级、浸润深度和淋巴结转移相关,差异有统计学意义(P<0.05),maspin与组织学分级、分期和淋巴结转移相关,差异有统计学意义(P<0.05).Twist和maspin没有显著相关(r=0.322,P=0.056).结论:在不典型子宫内膜增生和子宫内膜样腺癌组织中,Twist和maspin蛋白表达不同,表明它们在子宫内膜样腺癌发展中的潜在功能,可能与子宫内膜样腺癌发生有关.

摘要： BACKGROUND Diabetic retinopathy(DR)is a serious and potentially blinding complication of diabetes mellitus.Retinal neovascularization is one of the main pathological features of proliferative DR,and inhibiting retinal neovascularization is a research focus.AIM The aim was to evaluate the effect of intravitreal injection of recombinant human maspin on neovascularization in DR.METHODS An oxygen-induced retinopathy(OIR)mouse model was used to simulate neovascularization in DR.New born C57BL/6J mice were randomly divided to a normal control group,a maspin injection OIR group,and an OIR group.The mice in the maspin injection OIR group were injected with recombinant human maspin in the bilateral vitreous cavity on postnatal day P12,and those in the OIR group were injected with sterile phosphate buffered saline.The protein expression of vascular endothelial growth factor(VEGF)and hypoxia-inducible factor 1-alpha(HIF-1α)in the retina was measured by western blotting,and the mRNA expression of VEGF and HIF-1αwas measured by real-time polymerase chain reaction.The vascular cell nuclei that broke through the inner limiting membrane(ILM)were counted in haematoxylin-eosin stained retinal sections.RESULTS It was found that the number of vascular cell nuclei breaking through the ILM was 31.8±8.75 in the OIR group,which was significantly more than that in the normal control group(P<0.001).The number of vascular cell nuclei breaking through the ILM was 6.19±2.91 in the maspin injection OIR group,which was significantly less than that in OIR group(P<0.01).The relative protein and mRNA expression of VEGF and HIF-1αwas significantly lower in the retinas in the maspin injection OIR group than in those in the OIR group(P<0.01).CONCLUSION Maspin inhibited neovascularization in DR by modulating the HIF-1α/VEGF pathway,which provides a potential and effective strategy for the treatment of DR.

摘要： BACKGROUND Although the role of p53 in the evolution and prognosis of gastric cancer(GC)has been extensively examined,the exact mechanism of action is incompletely understood.In the last years,p53-target genes were supposed to be involved in the p53 pathway.One of them is the tumor-suppressor gene Maspin,which codifies the protein with the same name.Maspin activity depends on its subcellular localization.To our knowledge,the possible role of TP53 gene in Maspin subcellular localization,in GC cells,has not yet been studied in a large number of human samples.AIM To evaluate the possible role of wild-type and mutated p53 in Maspin subcellular localization.METHODS The present study included 266 consecutive patients with GC in which TP53 gene status,and mutations in exons 2 to 11,respectively,were analyzed and correlated with immunohistochemical expression of p53 and Maspin.RESULTS None of the 266 cases showed mutations in exon 9.The rate of TP53 mutations was 33.83%.The mutation rate was slightly higher in distally-located GCs,with a lower degree(≤5 buds/high power fields)of dyscohesivity(P<0.01).The wildtype cases had a longer survival,compared with mutant GCs,especially in patients without lymph node metastases,despite the high depth of tumor infiltration(P=0.01).The Dukes-MAC-like staging system was proved to have the most significant independent prognostic value(P<0.01).The statistical correlations proved that TP53 gene mutations in exon 7 might induce knockdown of Maspin,but wild-type p53 can partially restore nuclear Maspin expression and decrease the metastatic potential of gastric adenocarcinoma cells.CONCLUSION Downregulated Maspin might be induced by mutations in exon 7 of the TP53 gene but wild-type p53 can partially restore nuclear Maspin expression.These findings should be proved in experimental studies.

摘要： Maspin基因是一种肿瘤抑制基因,编码丝氨酸蛋白酶抑制剂.研究发现Maspin可诱导凋亡、抑制肿瘤细胞的侵袭和转移、抑制新生血管的形成从而发挥肿瘤抑制作用.近年来发现Maspin还与宿主的免疫相关.文章主要对Maspin的结构分布、肿瘤抑制作用机制及免疫相关性进行综述.

摘要： 目的探讨Survivin和Maspin在宫颈癌组织中的表达情况及其临床意义。方法应用免疫组织化学法检测65例宫颈癌组织、35例宫颈上皮内瘤样变(CIN)组织和35例正常宫颈组织中Survivin和Maspin的表达水平。结果Survivin在正常宫颈、CIN和宫颈癌组织中的阳性表达率分别为0.00%、51.43%和73.85%,Maspin则分别为80.00%、57.14%和27.69%。其中宫颈癌和CIN组织中Survivin阳性表达率均高于正常宫颈组织,且宫颈癌组织中Survivin阳性表达更显著;而宫颈癌组织中Maspin的阳性表达率低于CIN和正常宫颈组织,且CIN组织中Maspin的阳性表达率亦低于正常宫颈组织,差异均有统计学意义(P<0.05或0.01)。Survivin表达水平与宫颈癌组织学分化程度、FIGO分期和淋巴结转移有关(P<0.05),而Maspin表达水平则与宫颈癌FIGO分期和淋巴结转移有关(P<0.05或0.01)。结论Survivin和Maspin可能参与了宫颈癌的发生、发展过程,其表达水平的检测对分析、研判宫颈癌的预后评估及生物学行为有一定的辅助作用。

摘要： 目的 探讨Survivin和Maspin在宫颈癌组织中的表达情况及其临床意义.方法 应用免疫组织化学法检测65例宫颈癌组织、35例宫颈上皮内瘤样变(CIN)组织和35例正常宫颈组织中Survivin和Maspin的表达水平.结果 Survivin在正常宫颈、CIN和宫颈癌组织中的阳性表达率分别为0.00％、51.43％和73.85％,Maspin则分别为80.00％、57.14％和27.69％.其中宫颈癌和CIN组织中Survivin阳性表达率均高于正常宫颈组织,且宫颈癌组织中Survivin阳性表达更显著;而宫颈癌组织中Maspin的阳性表达率低于CIN和正常宫颈组织,且CIN组织中Maspin的阳性表达率亦低于正常宫颈组织,差异均有统计学意义(P＜0.05或0.01).Survivin表达水平与宫颈癌组织学分化程度、FIGO分期和淋巴结转移有关(P＜0.05),而Maspin表达水平则与宫颈癌FIGO分期和淋巴结转移有关(P＜0.05或0.01).结论 Survivin和Maspin可能参与了宫颈癌的发生、发展过程,其表达水平的检测对分析、研判宫颈癌的预后评估及生物学行为有一定的辅助作用.

摘要： 本发明涉及一种Maspin基因的原位杂交检测试剂盒，包括杂交探针、标记物，所述的杂交探针序列如SEQ ID NO.1所示。本发明还提供了一种Maspin基因原位杂交检测方法。另外，本发明还提供了试剂盒在制备检测乳腺癌疾病药物中的应用。本发明优点在于：本发明提供的试剂盒具有灵敏度高、特异性强的特点。本发明的检测方法操作方便、简单，能在区级以上医院普遍使用和推广。

摘要： 本发明涉及一种基于定量PCR检测maspin基因甲基化状态的方法及其应用，首先确定maspin基因编码序列上游调控区域的CpG岛的具体位点和序列信息，设计10对检测maspin编码序列上游调控区域中CpG岛甲基化状态的特异性引物。利用该引物特异性的扩增maspin编码序列上游调控区域中包含CpG岛的不同长度的序列，进行后续甲基化定量检测。该方法可检测乳腺癌患者乳腺癌组织或者乳腺癌转移部位maspin编码序列上游调控区域中CpG岛甲基化状态，诊断该病人是否已经发生乳腺癌转移、恶性程度等，辅助临床上对乳腺癌分期分型及治疗；也可检测正常人乳腺maspin编码序列上游调控区域中CpG岛甲基化状态，判断其发病几率、是否会发生乳腺癌转移、恶性程度等，辅助乳腺癌早期筛查，具有良好的应用前景。

摘要： 本发明公开了一种联合检测基因maspin和miR‑17诊断乳腺癌的试剂盒，属于疾病诊断领域。本发明通过研究发现maspin与乳腺癌呈负相关，miR‑17与乳腺癌呈正相关，联合检测这两个基因对乳腺癌进行诊断有很高的准确率。本发明的试剂盒包含检测基因maspin与miR‑17表达的引物，以及检测内参基因表达的引物，还包含实时定量PCR反应试剂；所述引物的序列见SEQ ID NO.1‑8。使用本发明试剂盒进行检测，当maspin的Ct值大于31，miR‑17的Ct值低于12时，可初步诊断为乳腺癌。本发明从基因水平上诊断乳腺癌比传统的提取被检测者的组织更精确而且减轻了患者的痛苦。