载脂蛋白C3促进THP-1细胞向小鼠主动脉黏附

摘要

目的 探讨载脂蛋白C3(APOC3)对THP-1细胞与小鼠主动脉黏附效应的影响.方法 在无菌环境下应用外科显微技术分离C57BL/6小鼠主动脉,在体外给予APOC3刺激16 h后与标记有CFSE荧光的单核细胞来源THP-1细胞黏附1h,观察黏附效应的变化,同时应用免疫组化方法检测血管黏附分子1(VCAM-1)和细胞间黏附分子1(ICAM-1)的表达.结果 给予APOC3刺激的主动脉与对照主动脉相比,与THP-1细胞的黏附效应增加,VCAM-1和ICAM-1表达上调,且前者比后者上调显著.结论 载脂蛋白C3促进THP-1细胞向小鼠主动脉黏附.%Objective To investigate the enhancing effect of apolipoprotein C3 (APOC3) on THP-1 cell adhesion to aortas of mice.Methods Microsurgery was performed to separate the aorta of C57BL/6 mice in sterile condition.After stimulated by APOC3 (100 △g/ml) in vitro for 16 h,the aorta was allowed to adhere for 1 h with CFSE labeled THP-1 cells (1 ×106/ml).Then the adhesion effect was observed,and the expressions of vascular adhesion molecule 1 (VCAM-1) and intercellular adhesion molecule 1 (ICAM-1) were detected by immunohistochemical method.Results Adhesion effect of the mice aorta with THP-1 cells in the APOC3 stimulated group was stronger than the control group.Both the expressions of VCAM-1 and ICAM-1 in aortas were increased by APOC3,but the former was significantly up-regulated than the latter.Conclusion Apolipoprotein C3 could enhance THP-1 cell adhesion to aortas of mice.