粪便转铁蛋白检测在提高消化道出血检出率中的作用

摘要

Objective To compare the sensitivity, the specificity and the anti-jamming of several excrement occult blood experimental techniques. To evaluate the effect of transferrin (Tf) in the excrement in the digestive tract hemorrhage detection rate. Methods For 600 patients of clinical suspicious digestive tract hemorrhage, take their excrement specimen, using the chemical process (pyramidon semi-quantitative examination law) to detect hemoglobin (Hb), and using monoclonal antibody colloidal gold method to detect Hb and Tf. Results Finally the hemoglobin chemical process (hereafter refers to as chemical process) to detect upper gastrointestinal hemorrhage with the positive rate 57.3%, and the detection of hemorrhage of lower digestive tract's positive rate is 44.8%; Hemoglobin monoclonal antibody colloidal gold method (hereafter refers to as colloid gold law) to examine upper gastrointestinal hemorrhage with a positive rate 60.4%, under examination hemorrhage with positive rate 77.6%; transferrin monoclonal antibody colloidal gold method (hereafter refer to as transferrin law) to examine upper gastrointestinal hemorrhage with a positive rate 82.3%, examination hemorrhage of lower digestive tract with a positive rate 66.4%; The union examination law (hemoglobin and transferrin to be detected twice, once positive that is positive) examines upper gastrointestinal hemorrhage the positive rate is 90.8%, hemorrhage of lower digestive tract's positive rate is 97.6%. Conclusion Excrement transferrin has the high detection rate in the upper gastrointestinal hemorrhage; Hb and the Tf combined examination may obviously raise the digestive tract hemorrhagic disease's positive detection rate.%目的 比较几种粪便隐血实验方法的灵敏性、特异性和抗干扰性,评价转铁蛋白(Tf)在粪便转铁蛋白检测在提高消化道出血检出率中的作用.方法 对600例临床怀疑消化道出血病人的粪便标本分别用化学法(匹拉米洞半定量检测法)、检测血红蛋白(Hb)、单克隆抗体胶体金法检测Hb和Tf.结果 血红蛋白化学法(以下简称化学法)检测上消化道出血的阳性率为57.3％,下消化道出血的阳性率为44.8％；血红蛋白单克隆抗体胶体金法(以下简称胶体金法)检测上消化道出血的阳性率为60.4％,下化道出血的阳性率77.6％；转铁蛋白单克隆抗体胶体金法(以下简称转铁蛋白法)检测上消化道出血的阳性率为82.3％,下消化道出血的阳性率66.4％；联合检测法(血红蛋白与转铁蛋白两次同时检测,有一项阳性即为阳性)检测上消化道出血的阳性率为90.8％,下消化道出血的阳性率为97.6％.结论 粪便转铁蛋白检测在上消化道出血中有较高的检出率；Hb与Tf联合检测可显著提高消化道出血性疾病的阳性检出率.