子痫前期患者环氧合酶2基因-765G＞C和-1195G＞A多态性的研究

摘要

Objective To investigate the relationship between two polymorphisms immediately upstream of the cyclooxygenase 2 (COX2) gene and preeclampsia in a South West Han Chinese population.Methods Blood samples from 205 patients with preeclampsia and 276 normal pregnant women as controls from Han Chinese in Chengdu area were analyzed by polymerase chain reaction-restriction fragment length polymorphisms.Results G and A allele frequencies for-1195G＞A site were 48.54％ and 51.46％ in the patient group,respectively,and 40.40％ and 59.60％ in the control group,respectively.G and C allele frequencies for-765G＞C site were 94.15％ and 5.85％ in the case group,respectively,and 94.38％ and 5.62％ in the control group,respectively.The AA genotype and variant A allelic frequencies of the-1195G＞A SNP were significantly lower in patients with preeclampsia than in the control group (P＜0.05),and the odds ratio for the risk of preeclampsia was 0.665 (95％ CI:0.444-0.982) in women homozygous for the variant COX2 A allele (x2 =4.233,P=0.047).The genotype and allele frequencies of the-765G ＞C polymorphism in patients with preeclampsia and controls showed no significant differences (P＞0.05).Additional subgroup analyses (mild vs severe preeclampsia) of the two polymorphisms failed to reveal significant correlation for either genotypic or allelic frequencies.Furthermore,there was no significant association between the polymorphisms and blood pressure levels in the patient or control groups.Conclusion COX2-1195A homozygosity is associated with a decreased risk for preeclampsia in a South West Han Chinese population.On the other hand,the-765G＞C polymorphism has no effect.%目的 探讨环氧合酶2(cyclooxygenase 2,COX2)基因变异是否与子痫前期发病有关联.方法 采用聚合酶链反应-限制性片段长度多态性分析法对成都地区205例子痫前期患者和276名健康孕妇COX2基因-765G＞C和-1195G＞A多态性进行分析.结果 COX2基因-1195位点G、A等位基因的频率在子痫前期组为48.54％、51.46％,在正常孕妇组为40.40％、59.60％;-765位点G、C等位基因的频率在子痫前期组为94.15％、5.85％,在正常孕妇组为94.38％、5.62％.-1195G＞A位点基因型和等位基因频率在子痫前期组和正常妊娠对照组之间差异具有统计学意义(P＜0.05),AA基因型携带者在患者组的频率显著低于对照组(26.34％ vs 35.15％),AA基因型携带者发生子痫前期的风险降低(P=0.047,x2=4.233,95％ CI:0.444～0.982).进一步对轻度和重度子痫前期亚组进行分析,未见两组之间上述位点基因型和等位基因频率存在差异.此外,子痫前期组和正常妊娠对照组COX2基因两位点基因型对血压水平未见显著影响.结论 成都地区汉族人子痫前期和对照人群COX2基因-1195G＞A基因型和等位基因频率有差异,未见-765G＞C位点与子痫前期的发生有关.