滇黄精中两个呋甾皂苷的NMR研究

摘要

A pair of stereoisomeric furostanol saponins were isolated from fresh rhizomes of Polygonatum kingianum, and identified as 26-O-β-D-glucopyranosyl 25 (S)-furostane-5-en-3β, 22,26-triol-12-on-3-O-β-D-glucopyranosyl ( 1→ 4 )-β-D-fucopyranoside ( 1 ) and 26-O-β-D-glucopyranosyl 25 (R)-furostane-5-en-3β, 22, 26-triol-12-on-3-O-β-D-glucopyranosyl (1→ 4)-β-D- fucopyranoside (2), respectively, by 1D 1 H- and 13C-NMR and 2D NMR (including 1 H-1H COSY,HSQC and HMBC). 1H and 13C chemical shifts of the two compounds were completely assigned, and the configurations of the methyl group at C-25 were determined.%从滇黄精新鲜根茎中分离得到一对立体异构的呋甾皂苷,利用1D、2D NMR鉴定其结构为:26-O-β-D-吡喃葡萄糖基25(S)-呋甾-△5(6)-烯-3β,22,26-三羟基-12酮基-3-O-β-D-吡喃葡萄糖基(1→4)-β-D-吡喃呋糖苷(1,25S-kingianoside D)和26-O-β-D-吡喃葡萄糖基25(R)-呋甾-△5(6))-烯-3β,22,26-三羟基-12酮基-3-O-β-D-吡喃葡萄糖基(1→4)-β-D-吡喃呋糖苷(2,kingianoside D).利用1D、2D NMR对两个呋甾皂苷25位的立体构型进行了确定,并对其碳、氢信号进行了全归属.