CdTe量子点-罗丹明B荧光共振能量转移法测定溶菌酶

摘要

The water-soluble CdTe was synthesized using thioglycolic acid as stabilizer. The fluorescence resonance energy transfer (FRET) between the CdTe QDs and Rhodamine B (RhB) in the presence of cetyltrimethylammonium bromide (CTAB) was characterized, in which the QDs with an emission wavelength of 530 nm was used as energy donor and rhodamine B as acceptor. Lysozyme content in lysozyme buccal tablets was determined by fluorescence resonance energy transfer. The results showed that the extent of fluorescence energy transfer quenching was linearly proportional to the concentration of lysozyme from 2 × 10-7 mol · L-1 to 8 × 10-6 mol · L-1 with a correlation coefficient of 0. 991 0. The minimum detection concentration was 2 × 10 -8 mol · L-1, RSD and the average recovery was 5. 8% and 101% (n =5) , respectively.%合成了以硫代乙醇酸为稳定剂的CdTe量子点,以发射波长为530 nm的量子点为供体,罗丹明B为受体,建立一种以十六烷基三甲基溴化铵为介质的荧光共振能量体系检测溶菌酶含片中溶菌酶含量的方法.结果表明:在pH =5.0时,溶菌酶的浓度与共振能量转移效率降低值在2 × 10-7～8×10-6 mol·L-范围内呈线性关系,其线性方程为Y=306.07 - 13.85X,相关系数为0.9910,检出限为2×10-8 mol· L-1,RSD为5.8％,平均回收率为101％(n=5).