TRIB3基因rs2295490多态性与冠心病血脂异常的相关性研究

摘要

目的 探讨TRIB3基因rs2295490多态性与冠心病(CHD)血脂异常的关系.方法 病例对照研究.收集2013年10月至2014年10月于重庆医科大学附属永川医院心内科住院的220例冠心病患者作为观察组(平均年龄60.15岁,男性150例,女性70例),同时收集151名健康体检者做为对照组(平均年龄59.22岁,男性102名,女性49名),利用PCR和基因测序技术检测两组的TRIB3基因rs2295490多态性,同时对患者的生化指标如葡萄糖(GLU)、总胆固醇(TC)、甘油三酯(TG)、高密度脂蛋白(HDL-C)、低密度脂蛋白(LDL-C)进行检测,分析TRIB3基因rs2295490多态性在各组中的分布及各组不同基因型与生化指标的关系.各组间基因型和等位基因频率比较采用x2检验,生化指标间的比较采用独立样本t检验.结果 TRIB3基因rs2295490多态性在健康对照组中的分布是AA基因型频率为57％,AG为35％,GG为8％,A、G等位基因频率分别为75％、25％;CHD组AA、AG、GG基因型频率分别为50％、41％、9％,A、G等位基因频率分别是71％、29％.健康对照组和CHD组间基因型和等位基因分布频率差异没有统计学意义(x2=1.74,P=0.42;x2=1.26,P=0.243);CHD组AA基因型携带者的HDL-C水平明显高于AG+ GG基因型(t=-9.78,P=0.00),TG和LDL-C水平明显低于AG+ GG基因型(t=2.59,P=0.01;t =6.15,P=0.00).结论 TRIB3基因rs2295490多态性与CHD可能无相关性,但G等位基因可能与CHD患者的血脂代谢紊乱有关.%Objective To explore the correlation between the polymorphism of TRIB3 gene rs2295490 and coronary heart disease (CHD) with dyslipidemia.Methods In the case control study,we enrolled 220 CHD patients as observation group (60.15 years mean age,150 males and 70 females) and 151 age and gender matched healthy individuals as control group (59.22 years mean age,102 males and 49 fe males).Genotypes of TRIB3 gene rs2295490 polymorphisms in the two groups were detected by PCR and sequence-based testing.At the same time,blood glucose (GLU),total cholesterol (TC),triglyceride (TG),high density lipoprotein cholesterol (HDL-C),low density lipoprotein cholesterol (LDL-C) were measured.The correlations between the polymorphism of TRIB3 gene rs2295490 and CHD,biochemical indicators of two groups were analyzed,respectively.The comparison of genotype and allele frequency between two groups used x2 test,while the comparison of biochemical indexes between two groups used t test.Results In the control group,genotypic frequencies of AA,AG and GG of TRIB3 gene rs2295490 were 57％,35％ and 8％,and the frequencies of A and G alleles were 75％ and 25％,respectively.In the CHD group,genotypic frequencies of AA,AG and GG of TRIB3 gene rs2295490 were 50％,41％ and 9％,and the frequencies of A and G alleles were 71％ and 39％,respectively.There was no significant difference of genotype and allele frequencies of polymorphism of TRIB3 gene rs2295490 between CHD and healthy controls(x2 =1.74,P =0.42;x2 =1.26,P =0.243).In the CHD group,the HDL-C levels of the AA genotype were higher than those of AG + GG genotype (t =-9.78,P =0.00);The TG,LDL-C levels of the AA genotype were lower than those of AG + GG genotype (t =2.59,P =0.01;t =6.15,P =0.00).All the differences were statistically significant.Conclusions TRIB3 gene rs2295490 polymorphism has no correlation with CHD,but the G allele may be correlated with dyslipidemia in CHD patients.