锌对脊髓缺血再灌注损伤大鼠ATP酶、MAO活性和T-AOC含量影响的研究

摘要

Objective To study the effect of the exogenous zinc supplement on the ATP enzyme、MAO activity and T-AOC content of rats with spinal cord ischemia-reperfusion inj ury in order to explore the mechanism of protective effect of zinc on the spinal cord ischemia-reperfusion injury.Methods 60 Wistar rats were randomly divided into five groups:sham-operation group,model group,and zinc sulfate pre-treatment groups including high-dose zinc group (100 mg/kg),middle-dose zinc group (50 mg/kg)and low-dose zinc group(25 mg/kg),12 rats in each group,half male and half female.In all the zinc pre-treatment groups,rats were administered zinc sulfate one time by gavage in seven days succession.The rats in sham-operation group and model group were administered the same volume normal saline solu-tion as zinc pre-treatment group.Spinal cord ischemia-reperfusion inj ury model was established after zinc pretreatment for 7days.24 h after injury,Motor function assessment of rats were performed by Basso Beattie Bresnahan (BBB)Mo-tor Function Assessment;ATP enzyme,MAO activity and T-AOC content in spinal cord were detected.Results BBB motor function assessment of zinc group were significantly higher than that of model group (P<0.05).Na+-K+-ATP enzyme activity in zinc pre-treatment groups was significantly lower than that in sham-operation group(P<0.05),but significantly higher than that in model group(P<0.05).Ca2+-Mg2+-ATP enzyme activity in rats in model group and zinc pre-treatment groups was significantly lower than that in sham-operation group(P<0.05).MAO activity in rats in model group and zinc pre-treatment groups significantly higher than that in sham-operation group(P<0.05).And MAO activity in rats in high-dose zinc group (100 mg/kg)was significantly lower than that in model group (P<0.05). T-AOC activity in rats in model group and zinc pre-treatment groups was significantly lower than that in sham-opera-tion group (P<0.05),T-AOC content in model group was the lowest and increased with the increasing of zinc dose. Conclusion zinc has a protective effect on spinal cord ischemia-reperfusion inj ury by possibly.%目的：方法研究外源性补锌对脊髓缺血再灌注损伤大鼠 ATP酶、MAO活性和 T-AOC含量的影响，从而探讨锌对脊髓缺血再灌注损伤的保护作用的机制。方法60只 Wistar大鼠随机分为5组，每组12只，雌雄各半，分别为假手术组、模型组、硫酸锌预处理高剂量组（100 mg／kg）、中剂量组（50 mg／kg）和低剂量组（25 mg／kg）。各剂量组每日灌胃给予硫酸锌1次，连续7天。假手术组和模型组给予相同剂量生理盐水，7天后制备脊髓缺血再灌注损伤动物模型，损伤后24 h采用Basso Beattie Bresnahan（BBB）运动功能评分法对脊髓缺血再灌注损伤模型进行后肢神经功能评价；测定脊髓组织中 ATP酶、MAO活性和T-AOC含量。结果与模型组比较，各给锌组大鼠神经功能评分明显高于模型组，差异具有显著性（P＜0．05）。各硫酸锌处理组 Na＋-K＋-ATP酶活性明显低于假手术组（P＜0．05），但明显高于模型组（P＜0．05）。模型组和各硫酸锌处理组脊髓组织 Ca2＋-Mg2＋-ATP酶活性显著低于假手术组（P＜0．05）。模型组和各硫酸锌处理组大鼠脊髓组织内的 MAO活性均显著高于假手术组（P＜0．05），且100 mg／kg／d 硫酸锌处理组 MAO活性显著低于模型组（P＜0．05）。模型组和各硫酸锌处理组大鼠脊髓组织内的 T-AOC含量显著低于假手术组（P＜0．05）。模型组大鼠脊髓组织中T-AOC含量最低，随着硫酸锌剂量的增加，T-AOC含量呈上升趋势。结论锌可能通过提高ATP酶的活性、抑制MAO活性和升高T-AOC含量，从而对脊髓缺血再灌注损伤起到一定的保护作用。