结合基序蛋白6在结直肠癌组织中的表达及临床意义

摘要

目的 探讨RNA结合基序蛋白6(RBM6)在结直肠癌(CRC)中表达水平及临床病理学意义.方法 应用免疫组织化学方法检测92例配对的CRC及癌旁组织石蜡包埋标本RBM6表达水平,观察其与CRC病人临床病理学参数的关系;再以Western印迹和qRT-PCR检测28例配对冷冻保存的新鲜CRC及癌旁组织中RBM的蛋白和mR-NA表达水平.结果 免疫组化结果显示:RBM6在92例CRC表达明显高于癌旁组织表达水平(癌组织30.4％,28/92 vs癌旁10.9％,10/92;P＜0.01).卡方检验显示:RBM6的表达与CRC肿瘤的大小、分化程度、浸润深度及淋巴结转移无明显相关性(P＞0.05),但与患者TNM分期和远处转移呈正相关(P=0.026,P=0.021).单因素分析发现:RBM6高表达CRC患者预后更差(P=0.024).qRT-PCR和Western印迹结果显示:RBM6在CRC中蛋白和mRNA表达均高于配对癌旁组织(P＜0.01;P=0.009).结论 RBM6在CRC中呈高表达,并与患者临床分期、远处转移及不良预后相关,可能参与CRC的恶性进展.%Objective To investigate the expression and clinical significance of RNA binding motif protein 6(RBM6) in colorectal cancer tissue.Methods The expression of RBM6 protein in 92 paired paraffin embedded CRC specimens and adjacent non-cancerous colorectal tissues were detected by Immunohistochemistry.Western blot and quantificational RT-PCR (qRT-PCR) were used to examine the expression of RBM6 mRNA and protein levels in 28 paired fresh CRC specimens and adjuvant non-cancerous tissues.The relationship between the protein expression and clinicopathological features was analyzed.Results RBM6 protein was overexpressed in CRC than that in paired adjacent normal colorectal tissues(P＜0.01).High RBM6 expression was positively correlated with TNM stage and distant metastasis,respectively(P=0.026,P=0.021),but had no association with tumor size,differentiation,invasion depth and lymph metastasis (P＞0.05).CRC patients with RBM6 overexpression had a worse prognosis(P=0.024).Both protein and mRNA levels of RBM6 were higher in CRC than that in non-tumorous colorectal tissues detected by Western blot and qRT-PCR,respectively (P＜0.01;P=0.009).Conclusion RBM6 is overexpressed in CRC and is positively associated with TNM stage,distant metastasis and poor prognosis of CRC patients.Aberrant expression of RBM6 might participate in the aggressive progression of CRC.