复方鱼腥草合剂对db/db小鼠心肌损伤的影响

摘要

Objective To investigate the effects of different extracts from compound HerbaHouttuyniae on development of diabetic car-diomyopathy in db/db mice.Methods db/m mice were in the normal group,and db/db mice were divided into six groups according to their treatment ways randomly:model group,metformin hydrochloride group,water extract group,ethanol extract group,volatile oil group,and mixture group (mixture of above three extract)of compound herba houttuyniae (n=8 for each).The change of body weight and fasting blood glucose (FBG)of mice were detected. Fins,transforming growth factor beta 1 (TGF β1),fibronectin (FN),glucagon like peptide 1 (GLP 1)were measured by ELISA after treatment with 8 weeks.The structures of myocardial ultra-structure were observed under electron microscope.Results Compared with normal group,the serum level of TGF β1 ,FN in ethanol extract group,volatile oil group,and mixture group were decreased(P<0.05).The level of GLP 1 in treatment groups except met-formin hydrochloride group were increased(P<0.05).And the myocardial ultrastructure were improved especial y in mixture group. Conclusion The compound herba houttuynia can lessen the cardiac pathologicalchange,and the mechanism may related to the de-crease of the level of TGF β1 ,FN and the increase of GLP 1.%目的：探讨复方鱼腥草合剂(鱼腥草、牛蒡子)对糖尿病小鼠心肌超微结构的影响。方法选取8只 db/m小鼠为正常组；48只 db/db小鼠分为模型组、盐酸二甲双胍组、复方鱼腥草合剂水提物组、醇提物组、挥发油组及完整成分组,每组8只、给药8周,记录小鼠体重及血糖,8周后 Elisa试剂盒法检测血清胰岛素、转化生长因子(TGF β1)、纤连蛋白(FN)、胰高血糖素样肽1(GLP 1)水平,取心肌组织采用透射电镜法观察小鼠心肌组织超微结构的变化。结果与模型组比较,醇提物、挥发油、完整成分组血清 TGF β1,FN水平降低(P<0.05),GLP 1含量升高(P<0.05)。心肌超微结构中肌丝排列相对整齐,线粒体肿胀程度小,改善明显,水提物组改善不明显,且以完整成分组改变最为显著。结论复方鱼腥草合剂对糖尿病所致心肌损伤有一定改善作用,其保护机制可能与下调 TGF β1、FN水平,减少心脏胶原纤维沉积,诱导 GLP 1分泌有关。