红景天对AOPPs诱导小鼠足细胞转分化的影响

摘要

目的:探讨红景天(salidroside)对晚期氧化蛋白产物(AOPPs)诱导小鼠足细胞转分化的影响.方法:以小鼠永生性足细胞系为研究对象.给予200 μg/ml的AOPP刺激小鼠足细胞24 h,同时加入红景天(浓度分别为0.1 μmol、1 μmol、10 μmol、100 μmol、200 μmol)进行干预,采用Western blot 检测内质网应激标志性蛋白Grp78、CHOP以及平滑肌激动蛋白(α-SMA)、nephrin、podocin的表达水平.结果:200 μg/ml AOPP条件下小鼠足细胞表达高水平的Grp78、CHOP及α-SMA,表达低水平的nephrin和podocin;予不同浓度红景天作用后,足细胞中Grp78、CHOP及α-SMA的表达水平降低,nephrin和podocin的表达水平升高.结果表明红景天可部分逆转AOPPs的作用,且存在一定的剂量依赖性.结论:红景天对AOPPs诱导的足细胞Grp78、CHOP过度表达有很好的抑制作用,这可能是它减轻肾脏纤维化的机制之一.%Objective: To investigate the effect of Salidroside on Mouse Podocytes Epithelial - mesenchymal transition ( EMT ) induced by Advanced Oxidation Protein Products( AOPP ). Methods: Using mice glomerular podocyte cell line as an in vitro system,podocytes were stimulated by AOPPs( 200 μg/ml ) and Salidroside( 0. 1 μmol,1 μmol,10 μmol,100 μmol,200 μmol )for 24 hours. Then the cells were collected and expression of alpha - smooth muscle actin( α - SMA ), Grp78( glucose - regulated protein78 ), CHOP, nephrin and podocin were detected by Western blot. Results : Under AOPPs( 200 μg/ml ) condition, there were high expressions of Grp78,CHOP and α -SMA,and low expression of nephrin and podocin in mice podocyte cell. After the treatment with Salidroside( 0. 1μmol,l μmol,10 μmol,100 μmol,200 μmol ),the expressions of Grp78, CHOP and α - SMA were decreased and the expression of nephrin and podocin were increased in mice podocyte cell. The results showed that Salidroside could partly reverse the effect of AOPPs in a dose - dependent manner. Conclusion: Salidroside can attenuate renal fibrosis, and the protective mechanism may be partly through by regulating the over expressions of genes Grp78 and CHOP.