A nickel(H) complex [Ni(en)3](L)2-3H2O (HL=2-phenyl-4-selenazole carboxylic acid, C1oH7O2NSe, en= ethylenediamine), was synthesized and characterized by elemental analysis, Infrared radiation (IR) and thermal analysis. Its crystal structure was determined by single crystal X-ray diffraction method. The complex, C26H42N8NiO7Se2, crystallizes in the triclinic system, space group PI, with cell parameters: α=l. 15673(3) nm, b-1.217 55(3) nm, c=1.263 26(3) nm, α=86.096(2)°, 0=89.461(1)°, γ=69.321(l)°, cell volume: v=1.66046(7) nm3, Z=2, relative molecular mass: M, =795.29. The interaction between complex and DNA were studied by EtBr fluorescent probe. The antibacterial activity of the ligand and complex against six species of bacteria which are E. coli JMI09, E. coli DH5a, S. epidermidis, S. aureus, baumanii, S. viridans were tested respectively. Also the inhibition of two compounds against normal cell 293T and tumor cell RAW 264.7 were tested. CCDC: 803433.%由2-苯基-4-硒唑甲酸(HL,C10H7O2NSe)、硫酸镍和乙二胺(en)合成了相应的配合物[Ni(en)3](L)2·3H2O.用元素分析、红外光谱、热重分析等表征手段确定了配合物的组成;用单晶X-射线衍射测定了配合物的晶体结构.配合物C26H42N8NiO7Se2属于三斜晶系,空间群P(1)晶胞参数:a=1.156 73(3) nm,b=1.217 55(3) nm,c=1.263 26(3) nm,α=86.096(2)°,β=89.461(1)°,y=69.321(1)°,晶胞体积∶V=1.660 46(7) nm3,晶胞内结构基元数Z=2,式量Mr=795.29.用溴化乙锭荧光探针法研究了配合物与DNA的相互作用.考察了配体和配合物对大肠埃氏杆菌(E. coli JM109)、大肠杆菌(E. coli DH5α)、表皮葡萄球菌(S. epidermidis)、金黄色葡萄球菌(S. aureu.s)、鲍曼不动杆菌(baumanii)、草绿色链球菌(S. viridans)六种细菌的抗菌活性及对正常细胞293T和肿瘤细胞RAW 264.7的体外增殖抑制作用.
展开▼
