HPLC-ELSD测定肉苁蓉及管花肉苁蓉中半乳糖醇含量

摘要

目的 建立肉苁蓉及管花肉苁蓉中半乳糖醇含量的测定方法,为肉苁蓉质量标准的制定提供依据.方法 通过建立半乳糖醇的高效液相色谱含量测定方法,对20个批次的肉苁蓉和管花肉苁蓉中半乳糖醇进行分析.色谱条件:采用Alltech Pevail Carbohydrate ES色谱柱(4.6 mm×250 mm,5 μm),以乙腈-水(80∶20)为流动相,流速为1.0 mL/min;进样量为10 μL;蒸发光散射检测器漂流管温度为80 ℃,载气流速为3.0 L/min.结果 半乳糖醇在89.6～1 120 mg/L范围内峰面积对数值与进样量对数值有良好的线性关系(r＝0.999 0),方法检出限为15.998mg/L,定量限为79.903mg/L,肉苁蓉和管花肉苁蓉中半乳糖醇的平均回收率(n＝5)分别为100.34%和101.11%,RSD分别为1.72%和1.33%.结论 该方法简便准确,灵敏度高,重复性好,可以作为肉苁蓉的质量控制方法.%Objective To establish a method for the determination of galactitol in Cistanche deserticola and Cistanche tubulosa, and to provide evidence for the quality control of Cistanches Herba. Methods The contents of galactitol in 20 batches samples of Cistanche deserticola and Cistanche tubulosa were determined by HPLC-ELSD. The samples were separated by Alltech Pevail Carbohydrate ES column (4.6 mm×250 mm, 5 μm) with acetonitrile-water (80∶20) as mobile phase. The flow rate was 1.0 mL/min and the injection volume was 10 μL. Shift tube temperature of ELSD was set at 80 ℃ and the gas flow rate was 3.0 L/min. Results The linear range of galactitol was 89.6－1 120 mg/L (r＝0.999 0), method detection limit was 15.998 mg/L, limit of quantitation was 79.903 mg/L. The average recovery rate of galactitol in Cistanche deserticola and Cistanche tubulosa was 100.34% and 101.11% respectively with RSD of 1.72% and 1.33 % (n＝5). Conclusion The method is sensitive, repeatable and accurate, which can be used for the quality control of Cistanches Herba.