复方金铃四逆四物失笑散颗粒挥发油提取及羟丙基-β-环糊精包合工艺研究

摘要

Objective To optimize the extraction process of volatile oil and inclusion preparation of hydroxypropyl-β-cyclodextrin from compoundJinling Sini Siwu Shixiaosan Granules.Methods The orthogonal approach of L9 (34) was applied to investigate the volume of volatile oil extraction by 3 factors to obtain the optimum extraction conditions. With inclusion rate of volatile oil and yield of inclusion compound as comprehensive evaluation indexes, the optimum preparation conditions were discussed. The characterization of this prepared inclusion was analyzed by TLC.Results The optimum extraction conditions were as follows:the crude medicine with medicinal powder was shattered into 20 meshes;8 times the amount of water was added and was extracted for 8 h. The best inclusion conditions were as follows:the proportion of volatile oil to HP-β-CD was 1∶6, with inclusion temperature of 30℃, grinding time of 2 h. Analysis results of TLC showed that stable inclusion compound was formed from volatile oil and HP-β-CD.Conclusion The optimized extraction process and inclusion preparation are with stable quality and high feasibility.%目的：优选复方金铃四逆四物失笑散颗粒中挥发油提取工艺及羟丙基-β-环糊精(HP-β-CD)包合工艺。方法采用L9(34)正交试验,以挥发油提取量为评价指标,确定最佳提取工艺。以挥发油包合率和包合物收得率综合评分为评价指标,优选最佳包合工艺。采用薄层层析法(TLC)对包合物进行表征分析。结果最佳提取工艺：药材粉碎度为20目,加8倍量水,蒸馏提取8 h。最优包合工艺：挥发油与HP-β-CD投料配比为1∶6,包合温度30℃,研磨2 h。TLC图谱显示挥发油已被包合并形成稳定包合物。结论优选的提取和包合工艺质量稳定、可行性强。