胃溃疡肝郁证候大鼠模型的建立及其评价

摘要

目的：探讨胃溃疡肝郁证候动物模型的建立及电针的治疗作用。方法采用 Open-field 法选择60只SD大鼠，适应性喂养7 d，随机分为空白组、模型组、模型对照组和电针组，每组15只。除空白组外，其余3组均采用慢性不可预见性刺激合并醋酸烧灼法建立胃溃疡抑郁证大鼠模型。模型对照组继续慢性不可预见性刺激，电针组电针“肝俞”“梁丘”治疗，连续13 d。观察大鼠一般状态、旷场实验结果、溃疡指数，RT-PCR检测海马组织脑源性神经营养因子（BDNF），ELISA检测血清胃泌素（GAS）含量。结果实验第21日，除空白组外其余3组大鼠出现精神萎靡、四肢无力、被毛乏泽、食欲降低等表现。实验第34日，模型对照组大鼠一般状况表现较模型组严重，电针组大鼠一般状况明显改善，模型组大鼠水平和垂直穿格数较空白组显著减少（P＜0.01），与模型组比较，模型对照组大鼠水平和垂直穿格数显著减少（P＜0.05），电针组大鼠水平和垂直穿格数显著增加（P＜0.01）；模型组大鼠溃疡指数较空白组显著升高（P＜0.01），与模型组比较，模型对照组大鼠溃疡指数显著升高（P＜0.05），电针组大鼠溃疡指数显著下降（P＜0.01）；模型组海马组织 BDNF mRNA表达较空白组显著下降（P＜0.01），与模型组比较，模型对照组海马组织BDNF mRNA表达显著下降（P＜0.05），电针组海马组织BDNF mRNA表达组显著升高（P＜0.01）；模型组大鼠GAS含量较空白组显著升高（P＜0.01），与模型组比较，模型对照组大鼠GAS含量显著升高（P＜0.05），电针组大鼠GAS含量显著降低（P＜0.05）。结论通过复合方式建立病证结合胃溃疡肝郁证候动物模型可行，电针“肝俞”和“梁丘”对该模型有治疗作用。%Objective To discuss the establishment of gastric ulcer liver depression syndrome rat model and the therapeutical effects of electroacupuncture. Methods As the result of Open-field experimental method, 60 SPF male SD rats were selected as experimental animals. After 7 days of adaptive feeding, 60 rats were randomly divided into blank group, model group, model control group, and electoracupuncture group, 15 rats in each group. Except for the blank group, the other three groups were stimulated by chronic unpredictable stimulation to establish rat depression model. Then model control group was once again stimulated by chronic unpredictable stimulation for 13 days;electoracupuncture group was treated at Gan Shu (BL18) and Liang Qiu (ST34) acupoints for 13 days. General state of rats, Open-field experimental results and gastric ulcer index were observed. BDNF was measured by RT-PCR. GAS content was measured by ELISA. Results After modeling for 21 days, except for the blank group, the other three groups appeared listlessness, limb weakness, and fur dry and lose luster, appetites reduced, etc. After modeling for 34 days, compared with model group, model control group had more severe general state than model group. After acupuncture treatment, the general state of acupuncture group was improved. The horizontal and vertical activity sore in model group decreased significantly compared with the blank group (P<0.01). Compared with the model group, the horizontal and vertical activity sore in model control group decreased significantly (P<0.05), and increased significantly in the electroacupuncture group (P<0.01). Ulcer index in the model group increased significantly compared with the blank group (P<0.01). Ulcer index in the model control group increased significantly compared with the model group (P<0.01). Ulcer index in electroacupuncture group decreased significantly (P<0.01). BDNF mRNA expression in hippocampus of model group decreased significantly compared with the blank group (P<0.01). Compared with model group, the BDNF mRNA expression in hippocampus of model control group decreased significantly (P<0.05). The BDNF mRNA expression in the electroacupuncture group increased significantly (P<0.01). The GAS content in the model group increased significantly compared with the blank group (P<0.01). Compared with the model group, the GAS content in the model control group increased significantly (P<0.05). The GAS content in the electroacupuncture group decreased significantly (P<0.05). Conclusion The establishment of depressive rat gastric ulcer model which combining disease and syndrome together proves the feasibility of this animal experimental model. Electroacupuncture for Gan Shu (BL18) and Liang Qiu (ST34) can improve the efficacy of this model.