Construction of Rat Model of Hepatic Fibrosis with Blood Stasis Syndrome Integrated with Traditional Chinese Medicine （TCM） Syndrome and Western Medicine Disease

摘要

[Objective]According to the theory of blood stasis in traditional Chinese medicine( TCM),the animal modeling method of hepatic fibrosis integrated with pathology and symptoms was explored and improved,to construct a new type of rat model of hepatic fibrosis with blood stasis syndrome integrated with traditional Chinese and western medicine. [Method]The hepatic fibrosis model of blood stasis with blood stasis syndrome was constructed by jointing multi factors,including intragastric administration of ethanol,high fat and low protein feeding,joint injection of dimethylnitrosamine( DMN),bovine serum albumin( BSA) and norepinephrine( NE). The modeling method was further compared with traditional CC14 single-factor modeling method from the aspects of mortality,blood stasis syndrome of TCM,syndrome grading,general morphology of liver pathology,liver function changes,as well as expression levels of three kinds of collagen( type I and type III collagen,α-SMA) determined by immunohistochemical staining method,and four items of rat hepatic fibrosis( HA,P3 NP,LN,CⅣ content) determined by radio enzyme immunoassay. [Result]In blood stasis group,(1) the mortality of rats was 20%;(2) model rats appeared typical blood stasis syndromes of TCM such as ecchymosis,dark purple tongue,loose stool,and blood stasis syndrome grading was high;(3) fibrosis changes of liver such as dark white surface,dense gray nodules and brittle texture were observed in general morphological examination;(4) serological liver function tests found that ALT and AST of model rats,as well as TBIL,DBIL and IBIL contents increased significantly;(5) immunohistochemical staining demonstrated that the expression levels of three kinds of collagen(type I and type III collagen,α-SMA) increased significantly;(6) four items of rat serum hepatic fibrosis,HA,P3 NP,LN,CⅣ content,increased significantly;(7) the above results in blood stasis syndrome model group(except morality and liver function) were higher than those in CC14 modeling group,and the difference was statistically significant( P < 0. 05). [Conclusion]The new improved modeling method effectively reduces high mortality in traditional CC14 modeling method.In addition to low mortality,the model animal has dual characteristics of disease in western medicine and syndrome in TCM. It is consistent with the pathological characteristics of hepatic fibrosis in western medicine when according with the basic characteristics of blood stasis syndrome in TCM.