卫气虚相关血浆代谢标志物的季节性变化研究

摘要

目的：观察春、夏、秋、冬不同季节卫气虚证 SD 大鼠的血浆代谢组学特征，寻找与卫气虚相关的物质基础，探讨卫气虚的本质。方法分别于春分日、夏至日、秋分日、冬至日前7 d适应性喂养SD雄性大鼠各20只，与各季节气候同步控制条件，按随机数字法将大鼠分为实验组和对照组。实验组通过疲劳加寒热交替法复制卫气虚大鼠模型。于各节气当日12：00取血，采用高效液相色谱-质谱联用仪检测血浆代谢物，通过偏最小二乘判别分析对数据进行统计分析，比较各季节实验组和对照组的数据和图谱，探寻体现组间差异的代谢物，推测与卫气虚相关的生物标志物。结果在春、夏、秋、冬不同的季节中，各实验组和对照组大鼠间代谢谱呈现明显差异。在4组比较中，同型半胱氨酸在春、夏、秋季实验组与对照组比较差异显著，神经酰胺在夏、冬季实验组与对照组比较差异显著，睾酮在春、夏季实验组与对照组比较差异显著，环鸟甘酸在秋、冬季实验组与对照组比较差异显著，肌氨酸在春、夏、冬季实验组与对照组比较差异显著，这些物质的生理功能涉及机体的脂代谢、氨基酸代谢、神经递质以及激素水平的调节。结论伴随季节变化，卫气虚的生物标志物也发生相应变化，主要体现在氨基酸代谢、脂类代谢、神经递质以及激素调节水平等方面。%Objective To study the plasma metabolomics features of SD rats with wei-qi deficiency respectively in spring, summer, autumn and winter; To seek the potential markers related to wei-qi deficiency; To discuss the essence of wei-qi deficiency.MethodsTwenty male SD rats were adaptively fed 7 days before Chinese Vernal Equinox Day, Summer Solstice, Autumn Equinox and Winter Solstice, synchronous controled with temporal condition. The rats were randomly divided into experimental and control groups according to random number method. Rats in the experimental groupwere made into wei-qi deficiency models through fatigue stimulation alternately with cold and heat stimulation. Blood was collected at 12:00 each solar term. High performance liquid chromatography- mass spectrometry instrument was used to detect plasma metabolites. Partial least squates-discriminant analysis was used to process statistical analysis of the data to compared the original data of plasma metabolomics between the two groups of each season, and explore the difference metabolic markers between each two groups, then speculate the potential biomakers of wei-qi differency.ResultsWith the changes of spring, summer, autumn and winter, significant differences were showed in metabolic profile between the experimental and control groups. Homocysteine showed a significant difference in spring, summer and autumn; Ceramide showed a significant difference in summer and winter; Testosterone showed a significant difference in spring and summer; Cyclic guanosine monophosphate showed a significant difference in autumn and winter; Sarcosine showed a significant difference in spring, summer and winter. The physiological functions of these substances related to lipid metabolism, amino acid metabolism, neurotransmitter and hormone regulation.ConclusionPotential biomakers of wei-qi deficiency changed along with the seasonal variation, which mainly reflected in lipid metabolism, amino acid metabolism, neurotransmitter and hormone regulation.