电针对血管性认知障碍大鼠学习记忆能力及脑组织炎症因子的影响

摘要

目的 观察电针对血管性认知障碍(VCI)大鼠学习记忆能力和大脑皮层炎症因子的影响,探讨针刺防治VCI的作用机制.方法 采用颈内动脉微栓子体外注入法制备VCI大鼠模型.将成模大鼠随机分为模型组、阳性药组和电针组,同时取正常大鼠作为对照组.造模3 d后,阳性药组给予盐酸多奈哌齐药液灌胃,电针组针刺"百会""足三里".治疗结束后,采用Morris水迷宫检测大鼠学习记忆能力,ELISA检测大鼠脑组织肿瘤坏死因子-α(TNF-α)、白细胞介素(IL)-6、IL-1β的含量.结果 定位巡航实验随训练次数的增加,阳性药组和电针组大鼠寻找平台平均逃避潜伏期较模型组均有不同程度的缩短;空间探索实验阳性药组和电针组较模型组首次穿越平台所需时间显著缩短;与对照组比较,模型组TNF-α、IL-6、IL-1β含量显著升高;与模型组比较,阳性药组和电针组TNF-α、IL-6、IL-1β含量均显著降低.结论 电针"百会""足三里"可能通过降低VCI大鼠大脑皮层TNF-α、IL-6和IL-1β的含量,改善大鼠学习记忆能力.%Objective To observe the effects of electroacupuncture on the learning and memory ability and cerebral cortex inflammatory factor of rats with vascular cognitive impairment (VCI); To discuss the mechanism of electroacupuncture for preventing and treating VCI. Methods VCI rat models were made in microemboli injection through internal carotid artery method. The successful modeled rats were randomly divided into model group, positive medicine group and electroacupuncture group, and normal rats were taken as control group. Three days after rat models were established, the positive medicine group was given donepezil hydrochlorideby gavage, and electroacupuncture group was given electroacupuncture at "Baihui" and "Zusanli" acupoints. After treatment, the learning and memory ability was detected by Morris water maze test. The contents of TNF-α, IL-6 and IL-1β in rat brain tissue were detected by ELISA. Results The water maze results showed that with the increase of the number of training, the average escape latency of rats to find platform in positive medicine group and electroacupuncture group all had different degrees of shortening in positioning cruise experiment; in space exploration experiment, positive medicine group and electroacupuncture group to cross the platform area for the first time were significantly reduced compared with the model group; compared with the control group, the contents of TNF-α, IL-6 and IL-1β in the model group were increased significantly; compared with the model group, the contents of TNF-α, IL-6 and IL-β in postive medicine group and electroacupuncture group were decreased. Conclusion Electroacupuncture at "Baihui"and "Zusanli" acupoints can decrease the contents of TNF-α, IL-1 and IL-6 in the cortex of VCI rats, and improve the learning and memory ability of rats.