工作场所空气中七氟烷、异氟烷、恩氟烷气相色谱测定方法

摘要

目的 建立工作场所空气中七氟烷、异氟烷、恩氟烷溶剂解吸气相色谱测定的方法.方法 空气中的七氟烷、异氟烷、恩氟烷采用活性炭管采集,二氯甲烷解吸,经100%二甲基硅氧烷(DB-1)毛细管色谱柱分离的气相色谱测定方法.结果 本方法七氟烷、异氟烷、恩氟烷质量浓度分别在1.9~304.8 μg/ml、2.1~300.4 μg/ml和1.7~305.2 μg/ml范围内线性关系良好,相关系数均大于0.999,检出限分别为0.6、0.6和0.5μg/ml,定量下限分别为1.9 μg/ml、2.1μg/ml和1.7μg/ml,方法最低检出质量浓度分别为0.1、0.2、0.1 mg/m(3 按采集空气样品4.5 L计算);七氟烷、异氟烷、恩氟烷的相对标准偏差分别为2.5%~3.0% 、2.3%~3.1% 、2.2%~3.0% ;平均解吸效率分别为 101.1%~103.3% 、100.7%~102.7% 、101.0%~102.9%;七氟烷、异氟烷、恩氟烷穿透容量分别大于3.7、3.4、3.4 mg (100 mg活性炭);采样效率均为100.0%;样品在室温下至少可保存10 d.结论 本方法检出限低,准确度高,精密度好,适用于工作场所空气中七氟烷、异氟烷、恩氟烷的测定.%Objective To establish a solvent desorption gas chromatographic method for determination of Sevoflurane,Isoflurane and Enflurane in the air of the Workplace. Methods Sevoflurane,Isoflurane and Enflurane were collected with activated carbon tube and desorbed with dichloromethane,separated with DB-1 capillary columns, and then detected with flame ionization detector. Results The linearity ranges were 1.9-304.8 μg/ml for Sevoflurane, 2.1-300.4 μg/ml for Isoflurane and 1.7-305.2 μg/ml for Enflurane, The correlation coefficient was both﹥0.999. Their limits of detection were 0.6 μg/ml, 0.6 μg/ml and 0.5 μg/ml, and Their limits of quatification were 1.9 μg/ml, 2.1 μg/ml and 1.7 μg/ml, and their minimum detectable concentrations were 0.1﹑0.2 and 0.1 mg/m3 per 4.5 L of air. Their relative standard deviations ﹙RSD﹚were 2.5%-3.0%, 2.3%-3.1% and 2.2%-3.0%. The average desorption efficiencies were 101.1%-103.3%,100.7%-102.7% and 101.0%-102.9%. The sampling efficiency was both 100%. The breakthrough volume of 100 mg actived carbon was 3.7 mg,3.4 mg and 3.4 mg. Sevoflurane, Isoflurane and Enflurane in activated carbon tube could be kept at least 10 days at room temperature without significant losses. Conclusion The method shows lower detection limit, high accuracy and precision. It is feasible for determination of Sevoflurane, Isoflurane and Enflurane in the air of workplace.