石棉肺患者血清差异表达蛋白质筛选

摘要

目的 分离和鉴定石棉肺患者、石棉接触正常人群及未接触石棉正常对照人群的血清差异表达蛋白质,以期进一步探索发病机制及筛选潜在生物标志物.方法 以石棉肺患者37人作为石棉肺组,以曾进行石棉作业的健康工人254人作为阴性对照组,以该地区未接触石棉的439人作为正常对照组.采用蛋白质组学方法对3组人群血清蛋白进行分离和鉴定.结果 获得各组人群血清蛋白质凝胶图谱,分析发现石棉肺组同正常对照组比较,差异点≥1.5倍有21个(上调12个、下调9个);石棉肺组同阴性对照组比较,差异点≥1.5倍有34个(上调18个、下调16个);阴性对照组同正常对照组比较,差异点≥1.5倍有32个(上调19个、下调13个).经质谱鉴定和搜库,蛋白质为补体因子、α1-抗胰蛋白酶等,功能涉及机体免疫、抗肿瘤等.结论 长期接触石棉对机体免疫功能造成较大影响,其中一些蛋白质可作为早期诊断及干预的候选生物标志物.%Objective To screen differently expressed proteins for serum biomarkers by studying serum proteome of population with asbestosis,population exposed to asbestos without asbestosis and population never exposed to asbestos,to further understand the mechanisms of asbestosis.Methods The subjects of present study included 37 patients with asbestosis,254 workers exposed to asbestos and 439 healthy controls.The 2-dimensional gel electrophoresis (2-DE) and matrix-assisted laser desorptiou/ionization time of flight tandem mass spectrometry (MALDI-TOF -MS/MS) were used to screen and identify the differentially expressed serum proteins among all subjects.ImageMaster6.0 software was utilized to analyze the differentially expressed proteins.Results Well-qualified gel images of serum proteome were obtained,21,34 and 32 differentially expressed spots were found between asbestosis and normal controls,between asbestosis and negative controls or between negative controls and normal controls,respectively.Differentially displayed proteins were identified as cytokines,α1-AT,L-ficolin,etc.Conclusion Exposure to asbestos for a long period could interfere with the immune system of workers exposed to asbestos,and some proteins may serve as the biomarkers for early diagnosis and intervention of asbestosis.