抗CD4抗体对SEB诱导的PBMC增殖反应的抑制作用机理

摘要

Objective: To explore the mechanism of anti-CD4 antibodies effect on SEB-induced proliferation of the peripheral blood mono-nuclear cells (PBMC). Methods: Using MTT method to observe anti-CD4 antibodies effect on PBMC proliferation in different conditions. Usingmorphologic, biochemisry and flow cytometer methods to detect CD4+ T cells apoptosis induced by arti-CD4 antibodies.Results: Both anti-CD4human/murine chimeric antihodies and murine McAbs could inhihit the SEB-induced PBMC proliferatuon. Anti-CD4 chimeric antibodies couldspecially induced CD4+ T cells apoptosis.Conchusion: Most important inhibitory effects of the anti-CD4 antibody appear to difrect at TCR-in-duced early activation signals, and their inhibitoryeffect of anti-CD4 chimeric antibodies is close related to the monocytes. The further cross-linking of anti-CD4 antibodies is importan for inducing CD4+ T cells apoptosis.%探讨抗CD4抗体在SEB诱导的外周血单个核细胞(PBMC)增殖反应中的作用机理。方法：采用MTT法，以SEB为刺激原，观测在不同条件诱导的PBMC增殖反应中抗CD4抗体的作用。采用形态学、生物化学、流式细胞仪等方法，检测抗CD4抗体诱导的CD4+T细胞凋亡。结果：抗CD4人-鼠嵌合抗体和鼠源性单抗对由SEB诱导的PBMC增殖均有抑制作rn用。嵌合抗体能特异性诱导CD4+T细胞发生凋亡。结论：抗CD4抗体的增殖抑制作用能直接作用于TCR诱导的早期活化信号，嵌合抗体的抑制作用强度与单核细胞的存在密切相关，抗CD4嵌合抗体的进一步广泛交联是诱导凋亡的关键。