用模式抗原OVA研究CTA1-DD／IgG佐剂的免疫调节作用

摘要

Objective:To determine the immunological effects of a new type adjuvant CTA 1-DD/IgG.Methods:Intranasal im-munization of BALB/c mice with OVA in combination with adjuvant CTA 1-DD or CTA1-DD/IgG.Blood samples were subjected to OVA-specific IgG Abs detection and IgG subclass profiles assessment using ELISA.Splenic plasma cells secreting anti-OVA Abs were detected by ELISPOT.Results:CTA1-DD/IgG showed stronger activity in inducing OVA-specific IgG Abs and splenic Abs-secreting cells compared with CTA1-DD.The enhancement of IgG1,IgG2a and IgG2b Abs were observed,and the ratio IgG2a/IgG1 were >1 in both groups.Conclusion: CTA1-DD/IgG can exert enhanced adjuvant effects compared with CTA 1-DD.Mixed IgG subclasses are induced,indicating a more balanced Th1/Th2 response.%目的：进一步研究新型佐剂CTA1-DD/IgG的免疫调节作用。方法：小鼠经鼻内免疫抗原（鸡卵清白蛋白OVA）联合佐剂CTA1-DD或CTA1-DD/IgG复合物，用ELISA检测血清中OVA特异性IgG抗体生成和IgG抗体亚类，用酶联免疫斑点试验检测脾脏抗体分泌细胞。结果：CTA1-DD/IgG佐剂在小鼠中诱导血清特异性IgG抗体及脾脏特异性抗体分泌细胞的能力均高于CTA1-DD佐剂；CTA1-DD/IgG或CTA1-DD作为佐剂联合OVA免疫后IgG1、IgG2a、IgG2b 抗体皆提高，且IgG2a/IgG1＞1。结论：CTA1-DD/IgG佐剂的免疫调节作用强于CTA1-DD，且能产生混合IgG抗体亚型，促进平衡的免疫应答。