马齿苋多糖对荷宫颈癌小鼠免疫刺激活性的研究

摘要

目的：研究马齿苋（Portulaca oleracea L.）多糖（POL-P3b）对宫颈癌小鼠免疫刺激作用的影响。方法：采用移植性U14宫颈癌小鼠模型，随机分为荷瘤对照组、环磷酰胺组（CTX）和POL-P3b低剂量[POL-P3b（L）]、高剂量[POL-P3b （H）]组。连续给药13 d后，测定其胸腺指数和脾脏指数，ConA诱导小鼠脾淋巴细胞转化试验检测POL-P3b对小鼠机体的细胞免疫功能；ELISA法检测脾细胞分泌细胞因子IL-2、IFN-γ、IL-10和IL-4的含量；免疫组化和Western blot法检测肿瘤组织吲哚胺2，3-二氧化酶（ IDO）的表达。结果：POL-P3b可提高荷瘤小鼠免疫器官重量及增强荷瘤小鼠细胞免疫功能。 POL-P3b能降低荷瘤小鼠脾细胞分泌的IL-4和IL-10水平，并能促进IL-2和IFN-γ的分泌；POL-P3b能抑制肿瘤组织中IDO的表达，且高剂量组效果更明显。结论：POL-P3 b抗肿瘤作用的机制可能与提高机体免疫功能、降低免疫逃逸有关。%Objective:To study the immune stimulation effect of Portulaca oleracea L.polysaccharide ( POL-P3b) on cervical tumor-bearing mice.Methods:Using graft U14 cervical cancer mouse model ,and all the mice were randomly divided into tumor-bearing control group,cyclophosphamide (CTX) group and purslane polysaccharide low (L) (POL-P3b(L)) group and high dose of POL-P3b (POL-P3b(H)) group.After continuous treating 13 d,the thymus index and spleen index were determined; cellular immune function in mice was evaluated by ConA inducing mice spleen lymphocyte transformation test.The cytokines content of IL-2,IFN-γ,IL-10 and IL-4 secreted by spleen cells was detected using ELISA method.Indoleamine 2,3-2 oxidase ( IDO) expression in tumor tissue was detected by immunohistochemistry and Western blot method.Results:POL-P3b could obviously improve immune organ weight of tumor-bearing mice and enhance the cellular immunity function.POL-P3b could reduce the level of IL-4 and IL-10 secreted by spleen cells , and could promote the secretion of IL-2 and IFN-γ.POL-P3b also could inhibit the expression of IDO in tumor tissues ,and the effect of high dose group was more obvious.Conclusion:The antitumor mechanism of POL-P3b might be related to increase the body′s immune function and reduce the immune escape.