EAE小鼠胸腺萎缩与疾病严重程度关系的初步探究

摘要

Objective:To study the relationship between atrophy of the thymus and disease severity in EAE.Methods:MOG35-55 peptide induced EAE in C57BL/6 mice and analyzed the relationship between the severity of EAE and thymic atrophy,Flow cytometry analysis was used to evaluate thymic CD4+CD8+DP cells,CD4+CD8-,CD4-CD8+SP cells in relation to the severity of the disease.Results:The number of thymocytes in mice with decreased tail tone was (20.25 ±3.49) ×106 ,hindlimb weakness(4.93 ± 0.85)×106,complete hindlimb paralysis(1.8 ±0.19) ×106,and forelimb and hindlimb paralysis(0.52 ±0.07) ×106,there were statistically significant differences between groups ( P<0.05 ).As the disease progresses, CD4+CD8+DP cells ratio decreased, CD4+CD8-,CD4-CD8+SP cell ratio increased,different disease groups was statistically significant difference (P<0.05).Conclusion: The atrophy of thymus was closely related to the severity of EAE.Migration of activated T cells in EAE may cause atrophy of thymus.%目的：研究EAE小鼠胸腺萎缩与疾病严重程度的关系。方法：MOG35-55肽免疫C57BL／6小鼠诱导EAE，分析EAE小鼠疾病严重程度与胸腺萎缩的关系，流式细胞分析胸腺CD4＋CD8＋双阳性细胞、CD4＋CD8－、CD4－CD8＋单阳性细胞与疾病严重程度的关系。结果：尾部无力小鼠胸腺淋巴细胞数为（20.25±3.49）×106个，双后肢无力小鼠胸腺淋巴细胞数为（4.93±0.85）×106个，双后肢瘫痪小鼠胸腺淋巴细胞数为（1.8±0.19）×106个，四肢完全瘫痪小鼠胸腺淋巴细胞数为（0.52±0.07）×106个，组间差异有统计学意义（P＜0.05）；EAE疾病越重，CD4＋CD8＋双阳性细胞在胸腺细胞中的比例越低，CD4＋CD8－、CD4－CD8＋单阳性细胞比例越高，不同疾病组间差异有统计学意义（ P＜0.05）。结论：EAE小鼠疾病严重程度与胸腺萎缩程度关系密切，EAE小鼠中活化T细胞的定向迁移可能导致胸腺萎缩。