目的：检测早期肝细胞癌患者血清中microRNA-199a／b-3p的表达变化，探讨在肝细胞癌诊断中的价值。方法：收集肝细胞癌患者血清标本30例、肝硬化患者血清标本30例、健康人血清标本30例。提取血清总RNA逆转录，应用荧光定量检测法检测血清中miRNA-199a／b-3p的表达变化，并与AFP进行比较。结果：肝细胞癌、肝硬化患者血清miRNA-199a／b-3p水平均显著低于健康对照组（ P＜0.05）；miRNA-199a／b-3p诊断肝硬化和早期肝细胞癌的准确度及阳性率均优于AFP。结论：血清miRNA-199a／b-3p表达变化对早期肝细胞癌和肝硬化的具有一定的诊断价值。%Objective:To analyze the expression levels of serum microRNA-199a/b-3p ( miR-199-3p) in hepatocellular carcinoma (HCC) patients and to explore whether miR-199a/b-3p could be a novel biomarker of early HCC.Methods: Patients with normal liver,liver cirrhosis and early HCC in clinic were included in this study.Blood samples of the patients were collected for analy-sis.Real time quantitative PCR technique was used to measure the expression level of serum miRNA-199a/b-3p.The AFP expression was got from clinical data.Results: Comparing with normal liver and liver cirrhosis patients, miRNA-199a/b-3p presented lower expressions in early HCC patients (P<0.05);the accuracy and positive rate of serum miR-199a/b-3p detection in cirrhosis and HCC were better than AFP detection.Conclusion:Data indicated that miRNA-199a/b-3p had diagnostic value for indicating liver cirrhosis and early HCC.
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