目的：通过检测初发Graves病（ GD）患者的IFN-γ、IL-6、IL-17和TGF-β1等4种细胞因子水平，研究Graves病的发病机制与细胞因子的关系。方法：对23例初发Graves病（ GD组）的患者和23名健康对照者（ NC组）的血清分别用ELISA法检测IFN-γ、IL-6、IL-17和TGF-β1浓度，用电化学发光法检测FT3、FT4和TSH水平，用放射受体分析法测定TrAb水平。结果：GD组与NC组之间年龄、性别均无统计学差异（ t＝0.3348，P＞0.05；χ2＝0.4107，P＞0.05）。 GD组IFN-γ、IL-6、IL-17和TGF-β1的浓度显著高于NC组，差异有统计学意义（ P均＜0.05）；4种细胞因子与FT3、FT4均呈正相关（ r＝值依次为：0.3246、0.4532、0.4310、0.4638；0.4132、0.4415、0.4362、0.4671； P 均＜0.05）；与 TSH 均呈负相关（ r＝值依次为－0.4588、－0.4828、－0.4451、-0.4930；P均＜0.05）。结论：IFN-γ、IL-6、IL-17和TGF-β1等4种细胞因子在初发GD中均高表达；可能共同参与了GD的发病过程，可作为GD免疫功能紊乱的评价指标。%Objective:To explore the role of cytokines in the pathogenesis of Graves′disease(GD),by detecting the levels of IFN-γ,IL-6,IL-17 and TGF-β1 in GD patients who were newly diagnosed.Methods:A total of 23 patients with new onset GD and 23 gender-and age-matched healthy controls were examined.The levels of serum IFN-γ, IL-6, IL-17 and TGF-β1 were measured by ELISA,FT3,FT4 and TSH levels were determined by ECL IA;TrAb levels were tested by RRA.Results: There were no significant difference among GD and NC group in sex and age match ( t=0.334 8 ,P>0.05;χ2=0.410 7 ,P>0.05 ).The levels of serum IFN-γ,IL-6,IL-17 and TGF-β1 in the GD group were significantly higher than the control group ( P<0.05 ) .Correlation analysis revealed that IFN-γ,IL-6,IL-17 and TGF-β1 were positively correlated with FT3,FT4(r=0.324 6,0.453 2,0.431 0,0.463 8;0.413 2,0.441 5, 0.436 2,0.467 1;P<0.05 ).Conclusion: IFN-γ, IL-6, IL-17 and TGF-β1 are highly expressed in the newly diagnosed GD patients.They play an important role in the pathogenesis of GD ,and provide helpful evaluation indices of immune dysfunction to Graves disease.
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