膜表达型InsB15－23 H－2 Kd dtSCT对NOD 小鼠1型糖尿病发病的影响

摘要

Objective:To illuminate the influence of InsB15-23 H-2Kd dtSCT to the morbidity of type 1 diabetes mellitus in NOD mice.Methods:An eukaryotic plasmid encoded membrane-expressed InsB15-23 H-2Kd dtSCT was inoculated into 3 weeks old female NOD mice subcutaneously and the blood sugar and morbidity of type 1 diabetes mellitus were monitored once a week.To illuminate the cellular mechanism of immunologic intervention of membrane-expressed InsB15-23 H-2Kd dtSCT to the course of type 1 diabetes mellitus in NOD mice,the mononuclear cell infiltration of islets was detected by tissue slice and the frequency of IGRP206 2-14 specific CTLs in PBMC was analyzed by FACs.Results: As compared with pcDNA3.1 (-) control ( 60%) and untreated NOD mice ( 80%) , mice immunized with InsB15-23 H-2Kd dtSCT exhibited low level of islet infiltration and low morbidity in 30 weeks old ( 9%) .But the frequency of IGRP206-214 specific CTLs in PBMC of 16 and 40 weeks old mice showed no difference.Conclusion:Membrane-expressed InsB15-23 H-2Kd dtSCT can protect NOD mice from type 1 diabetes mellitus in IGRP206-214 independent pattern.%目的：研究膜表达型InsB15－23 H－2Kd dtSCT对NOD小鼠1型糖尿病发病率的影响。方法：用InsB15－23 mdtSCT真核表达载体皮下免疫3周龄NOD 母鼠，连续监测NOD小鼠的血糖水平以判断小鼠的发病情况。通过连续切片对NOD小鼠胰岛单个核细胞浸润情况进行检测，并监测IGRP206－214特异性CTLs的变化，来阐明InsB15－23 mdtSCT分子影响1型糖尿病病程的细胞学机制。结果：InsB15－23 mdtSCT组小鼠30周龄时的发病率（9％）显著低于空质粒组（60％）和自然发病组（80％），胰岛单个核细胞浸润情况也较轻，但16和40周龄时两组小鼠外周血IGRP206－214特异性CTLs水平无差异。结论：InsB15－23 mdtSCT真核表达载体能降低NOD小鼠1型糖尿病发病率，并且这种免疫保护作用是IGRP206－214非依赖性的。