目的：在小鼠金黄色葡萄球菌性乳腺炎过程中，探讨脾脏内多种Th细胞亚群数量和相关细胞因子、转录因子的动态变化及其意义。方法：本研究首先建立S.aureus感染小鼠的乳腺炎模型，再利用流式细胞术和荧光定量PCR技术，分别检测脾脏中多种Th细胞亚群数量及相关因子mRNA水平。结果：在S.aureus感染小鼠乳房后的不同时间，脾脏内Th2细胞和Th17细胞占CD4＋淋巴细胞的比例分别显著增加（ P＜0.05），而Treg细胞比例则明显降低（ P＜0.05）。并且，与Th细胞分化、活化和效应相关的多种细胞因子及转录因子，均在转录水平有明显升高（ P＜0.05）。结论：乳腺炎不只是发生于乳腺局部的一种炎症反应，机体还能够调动脾脏的多种Th淋巴细胞亚群，以分泌细胞因子的方式，组织有效的乳腺抗S.aureus感染免疫应答。%Objective:To study the dynamics of T helper cell subsets and related cytokines and transcription factors and its sig -nificance in spleen in mice during experimentally induced Staphylococcus aureus mastitis .Methods: We firstly established a murine mastitis model by challenge with an inoculum of Staphylococcus aureus,and then,the number of each T helper cell subset and the mRNA level of their associated factors were assessed in spleen by flow cytometry and quantitative real -time PCR,respectively.Results:At the indicated time points after Staphylococcus aureus infection, the proportion of Th2 cells and Th17 cells in CD4+lymphocytes were significantly increased,respectively,whereas,the proportion of regulatory T cells was significantly decreased (P<0.05).Moreover,the mRNA expression of genes encoding cytokines and transcription factors involved in the differentiation and function of various T helper cells,was greatly elevated in spleen (P<0.05).Conclusion:Mastitis is not only a local inflammation of the mammary gland ,but also involved in various T helper cell subsets in spleen via pro-inflammatory cytokine production to mediate effective immune responses against Staphylococcus aureus infection in mastitis.
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