目的:观察五味子乙素对胃癌SNU-1细胞增殖和转移的影响,探讨其诱导胃癌SNU-1细胞凋亡的机制.方法:不同浓度五味子乙素组和顺铂组加入到SNU-1细胞中,作用24 h后,MTT法测定药物组对胃癌SNU-1细胞增殖的抑制作用,流式细胞术检测不同浓度的五味子乙素诱导胃癌SNU-1细胞凋亡率,Transwell法检测五味子乙素对SNU-1细胞侵袭的抑制作用,采用间接荧光标记法测定细胞内活性氧(ROS)水平的变化;Western blot法检测胃癌SNU-1细胞中Caspase-3、Bcl-2、Bax、E-cadherin、N-cadherin和MMP-9的表达.结果:与空白对照组比较,10、50和100μg/ml的五味子乙素和顺铂对胃癌SNU-1细胞生长的抑制作用明显,10、50和100μg/ml的五味子乙素作用胃癌SNU-1细胞后,细胞转移能力显著下降,10、50和100μg/ml的五味子乙素作用胃癌SNU-1细胞48 h后均能诱导胃癌SNU-1细胞凋亡;ROS水平随着五味子乙素的浓度增加而增加明显;10、50和100μg/ml的五味子乙素上调Caspase-3、Bax、E-cadherin的表达,下调Bcl-2、N-cadherin和MMP-9的表达.结论:五味子乙素可能是通过Caspase-3级联凋亡途径、线粒体途径和ROS水平提高来诱导胃癌SNU-1细胞凋亡,也可能通过破坏细胞间和基底膜黏附性及增加肿瘤细胞间的稳定性,来抑制胃癌SNU-1细胞的转移能力.%Objective:To investigate the related mechanism of salidroside B on proliferation and metastasis of Rhodiola glucoside glioma cell.Methods:The inhibitory rate of salidroside B on SNU-1 cells were determined using MTT method .The effect of salidroside B on inhibiting metastasis of SNU-1 cells was determined by transwell assay .Finally the effect of expression of related proteins of SNU-1 cells was discussed.The SNU-1 cells apoptosis rate was detected by flow cytometry .Results: The growth inhibitory rates of SNU-1 cells were increased with the increasing of dose of salidroside B (10,50,100 μg/ml),and there were significant differ-enced.Apoptosis flow cytometry test results showed that SNU-1 cells apoptosis rate was increased with the increasing of dose of salidroside B(10,50,100 μg/ml).ROS levels was increased with the increase of the concentration of salidroside with significant differ -ence.The expression of salidroside B on Caspase-3,Bax and E-cadherin of SNU-1 cells were increased,Bcl-2,N-cadherin and MMP-9 of SNU-1 cells were decreased.Conclusion:The apoptosis of SNU-1 cells may be through Caspase-3 pathway,mitochondrial pathway apoptosis cascade and the level of ROS by salidroside B , and the reduce of metastasis ability may also through the destruction of adhesion between the cells and basement membrane and increased the stability of tumor cells by salidroside B .
展开▼
