长链非编码BANCR通过激活Wnt/β-catenin信号通路调控黑色素瘤细胞迁移和侵袭行为

摘要

Objective:To investigate the effect of long non-coding BANCR on the migration and invasion of melanoma cells and its mechanism.Methods:qPCR was used to detect the expression of BANCR in melanoma patients and the relationship between clini-copathological data.Kaplan-Meier analysis the survival of melanoma patients with different expression of BANCR.Transwell invasion assay was used to detect the effect of BANCR on the invasive ability of melanoma cells.The effect of BANCR on the migration of melanoma cells was detected by scratch healing assay.Western blot was used to detect Wnt/β-catenin Signal pathway protein expression.Results:BANCR was highly expressed in melanoma,especially with the higher pathological stage or the lymph node metastasis.The higher expression of BANCR,the worse survival of melanoma patients.The inhibition of BANCR expression could reduce the invasion and migration ability of melanoma cells.The expression of β-catenin and c-myc protein in Wnt/β-catenin signaling pathway was down-regulated after silencing BANCR.Conclusion:Long non-coding BANCR was highly expressed in melanoma patients and was negatively correlated with survival time,it also regulates melanoma cell migration and invasion by activating the Wnt/β-catenin signaling pathway.%目的:探究长链非编码BANCR调控黑色素瘤细胞迁移和侵袭行为及其相关机制.方法:qPCR检测BANCR在黑色素瘤患者中的表达和临床病理特征间的关系.Kaplan-Meier法分析BANCR的表达与黑色素瘤患者生存之间的关系;Transwell侵袭实验检测BANCR对黑色素瘤细胞侵袭能力的影响;划痕愈合实验检测BANCR对黑色素瘤细胞迁移能力的影响;Western blot检测抑制BANCR后Wnt/β-catenin信号通路蛋白的表达情况.结果:BANCR在黑色素瘤中高表达,而且在淋巴结转移或病理分期越高的患者中越显著;BANCR的表达越高,黑色素瘤患者生存越差;抑制BANCR表达可以降低黑色素瘤细胞的侵袭和迁移能力;沉默BANCR后,Wnt/β-catenin信号通路中β-catenin和c-myc蛋白表达下调.结论:长链非编码BANCR在黑色素瘤患者中普遍存在高表达,而且与患者生存时间呈负相关,同时它也能通过激活Wnt/β-catenin信号通路调控黑色素瘤细胞迁移和侵袭能力.